| Title: | Glucosinolate induces transcriptomic and metabolic reprogramming in Helicoverpa armigera |
| Author(s): | Jagdale S; Tellis M; Barvkar VT; Joshi RS; |
| Address: | "Biochemical Sciences Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411008 Maharashtra India. GRID: grid.417643.3. ISNI: 0000 0004 4905 7788 Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India. GRID: grid.469887.c Department of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune, 411007 Maharashtra India. GRID: grid.32056.32. ISNI: 0000 0001 2190 9326" |
| DOI: | 10.1007/s13205-020-02596-5 |
| ISSN/ISBN: | 2190-572X (Print) 2190-5738 (Electronic) 2190-5738 (Linking) |
| Abstract: | "Glucosinolates protect plants from herbivory. Lepidopteran insects have developed resistance to glucosinolates which is well studied. However, the molecular effects of glucosinolate intake on insects are unexplored. To elucidate this, we performed transcriptomics and metabolomics of sinigrin-fed Helicoverpa armigera. Transcriptomics exhibits significant dysregulation of 2375 transcripts, of which 1575 are upregulated and 800 downregulated. Gene Ontology analysis of differentially expressed genes reveals that key hydrolases, oxidoreductases, and transferases are majorly affected. The negative impact of sinigrin is significant and localized in the endomembrane system and mitochondria. It also disturbs various biological processes such as regulation of protein metabolism and cytoskeletal organization. Furthermore, H. armigera putative myrosinase-like enzymes may catalyze the breakdown of sinigrin to allyl isothiocyanate (AITC). AITC targets the electron transport chain causing oxidative stress. KEGG pathway enrichment shows significant upregulation of oxidative phosphorylation, glutathione metabolism and amino acid metabolism. Activation of these pathways induces glutathione synthesis for sinigrin detoxification. Differential gene expression indicates upregulation of glutathione S-transferase and succinate dehydrogenase suggesting mitochondrial impact. Transcriptomics data correlated with metabolomics show changes in serine, methionine, ornithine, and other metabolite levels. It corroborates well with the transcript alterations supporting the increased glutathione production. Thus, our data suggest that sinigrin generates oxidative stress in H. armigera and insects alter their metabolic wiring to overcome sinigrin-mediated deleterious effects. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-020-02596-5" |
| Keywords: | Detoxification Glucosinolate Glutathione Mitochondria Oxidative stress; |
| Notes: | "PubMed-not-MEDLINEJagdale, Shounak Tellis, Meenakshi Barvkar, Vitthal T Joshi, Rakesh S eng Germany 2021/01/15 3 Biotech. 2021 Jan; 11(1):26. doi: 10.1007/s13205-020-02596-5. Epub 2021 Jan 3" |
