| Title: | Isolation and DNA sequence of the STE13 gene encoding dipeptidyl aminopeptidase |
| Author(s): | Anna-Arriola SS; Herskowitz I; |
| Address: | "Graduate Group in Genetics, University of California, Berkeley 94720" |
| ISSN/ISBN: | 0749-503X (Print) 0749-503X (Linking) |
| Abstract: | "We have isolated a mutant which exhibits partial constitutivity for a-specific gene expression in alpha cells. The wild-type gene was cloned and demonstrated to be allelic to the STE13 gene, which encodes the dipeptidyl aminopeptidase involved in processing of the alpha-factor prepropheromone. Thus, the mating defect of the ste13 mutations in alpha cells may result both from the production of incompletely processed alpha-factor and from the increased expression of a-specific genes. The STE13 open reading frame of 931 amino acids contains a putative membrane-spanning segment near its amino terminus and is 31% identical to a second yeast dipeptidyl aminopeptidase (DAP2). A null mutant of STE13 has been constructed: it is viable and sporulation-proficient" |
| Keywords: | "Amino Acid Sequence Base Sequence Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/*genetics *Genes, Fungal Mating Factor Molecular Sequence Data *Peptide Biosynthesis *Peptides Pheromones/*biosynthesis Saccharomyces cerevisiae/*genetics *Saccharomyces ce;" |
| Notes: | "MedlineAnna-Arriola, S S Herskowitz, I eng AI18738/AI/NIAID NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1994/06/01 Yeast. 1994 Jun; 10(6):801-10. doi: 10.1002/yea.320100610" |
