| Title: | Octopamine neuromodulation regulates Gr32a-linked aggression and courtship pathways in Drosophila males |
| Author(s): | Andrews JC; Fernandez MP; Yu Q; Leary GP; Leung AK; Kavanaugh MP; Kravitz EA; Certel SJ; |
| Address: | "Center for Structural and Functional Neuroscience, University of Montana, Missoula, Montana, United States of America. Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, United States of America. Center for Structural and Functional Neuroscience, University of Montana, Missoula, Montana, United States of America; Division of Biological Sciences, University of Montana, Missoula, Montana, United States of America" |
| DOI: | 10.1371/journal.pgen.1004356 |
| ISSN/ISBN: | 1553-7404 (Electronic) 1553-7390 (Print) 1553-7390 (Linking) |
| Abstract: | "Chemosensory pheromonal information regulates aggression and reproduction in many species, but how pheromonal signals are transduced to reliably produce behavior is not well understood. Here we demonstrate that the pheromonal signals detected by Gr32a-expressing chemosensory neurons to enhance male aggression are filtered through octopamine (OA, invertebrate equivalent of norepinephrine) neurons. Using behavioral assays, we find males lacking both octopamine and Gr32a gustatory receptors exhibit parallel delays in the onset of aggression and reductions in aggression. Physiological and anatomical experiments identify Gr32a to octopamine neuron synaptic and functional connections in the suboesophageal ganglion. Refining the Gr32a-expressing population indicates that mouth Gr32a neurons promote male aggression and form synaptic contacts with OA neurons. By restricting the monoamine neuron target population, we show that three previously identified OA-Fru(M) neurons involved in behavioral choice are among the Gr32a-OA connections. Our findings demonstrate that octopaminergic neuromodulatory neurons function as early as a second-order step in this chemosensory-driven male social behavior pathway" |
| Keywords: | "*Aggression Animals Animals, Genetically Modified Base Sequence Behavior, Animal/*physiology DNA Primers Drosophila/*physiology Drosophila Proteins/genetics/*physiology Male Neurons/*physiology Octopamine/*physiology Polymerase Chain Reaction Receptors, C;neuroscience;" |
| Notes: | "MedlineAndrews, Jonathan C Fernandez, Maria Paz Yu, Qin Leary, Greg P Leung, Adelaine K W Kavanaugh, Michael P Kravitz, Edward A Certel, Sarah J eng R01 GM067645/GM/NIGMS NIH HHS/ P20 RR015583/RR/NCRR NIH HHS/ P20 RR017670/RR/NCRR NIH HHS/ GM0067645/GM/NIGMS NIH HHS/ R01 GM074675/GM/NIGMS NIH HHS/ GM074675/GM/NIGMS NIH HHS/ 2P20RR017670/RR/NCRR NIH HHS/ P20RR015583/RR/NCRR NIH HHS/ R01 GM099883/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2014/05/24 PLoS Genet. 2014 May 22; 10(5):e1004356. doi: 10.1371/journal.pgen.1004356. eCollection 2014 May" |
