| Title: | CRISPR/Cas9 Editing of the Codling Moth (Lepidoptera: Tortricidae) CpomOR1 Gene Affects Egg Production and Viability |
| Author(s): | Garczynski SF; Martin JA; Griset M; Willett LS; Cooper WR; Swisher KD; Unruh TR; |
| Address: | "USDA-ARS, Temperate Tree Fruit and Vegetable Research Unit, Wapato, WA 98951. Department of Chemistry, University of Connecticut, Storrs, CT. Department of Biology, University of Southern California, Los Angeles, CA" |
| ISSN/ISBN: | 1938-291X (Electronic) 0022-0493 (Linking) |
| Abstract: | "The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is a major pest of pome fruit worldwide. Incorporation of semiochemicals, including the main sex pheromone (codlemone), into codling moth IPM programs has drastically reduced the amount of chemical insecticides needed to control this orchard pest. Odorant receptors located in sensory neuron membranes in the antennae are key sensors in the detection of semiochemicals and trigger downstream signaling events leading to a behavioral response. CpomOR1 is an odorant receptor belonging to the pheromone receptor subfamily in codling moth, and is a prime candidate for being a codlemone receptor based on its high expression levels in male antennae. In this study, the CpomOR1 gene was targeted using CRISPR/Cas9 genome editing to knockdown functional OR1 protein production to determine physiological function(s). By injecting early stage eggs, mutations were successfully introduced, including both deletions and insertions. When attempting to create stable populations of codling moth through mating of males with females containing mutations of the CpomOR1 gene, it was found that fecundity and fertility were affected, with edited females producing nonviable eggs. The role of CpomOR1 in fecundity and fertility in codling moth is unknown and will be the focus of future studies" |
| Keywords: | Animals Arthropod Antennae/metabolism *CRISPR-Cas Systems Female Gene Editing Insect Proteins/*genetics/metabolism Male Moths Ovum/metabolism Reproduction CRISPR/Cas9 genome editing odorant receptor; |
| Notes: | "MedlineGarczynski, Stephen F Martin, Jessica A Griset, Margaret Willett, Laura S Cooper, W Rodney Swisher, Kylie D Unruh, Thomas R eng England 2017/09/01 J Econ Entomol. 2017 Aug 1; 110(4):1847-1855. doi: 10.1093/jee/tox166" |
