| Title: | The expression of nerve growth factor in mice lung following low-level toluene exposure |
| Author(s): | Fujimaki H; Tin Tin Win S; Yamamoto S; Nakajima D; Goto S; |
| Address: | "National Institute for Environmental Studies, Tsukuba, Ibaraki, 305-8506, Japan. fujimaki@nies.go.jp" |
| DOI: | 10.1016/j.toxlet.2009.09.004 |
| ISSN/ISBN: | 1879-3169 (Electronic) 0378-4274 (Linking) |
| Abstract: | "To clarify the effect of indoor air pollutants on nerve growth factor (NGF) production in lung, male C3H/HeN mice were exposed to filtered air (control) or toluene at levels of 0.9 ppm, 9 ppm, or 90 ppm for 30 min via nose-only inhalation on days 0, 1, 2, 7, 14, 21, 28, 35, 42, 49 and 56. As an allergic mouse model, some mice (n=24) were immunized with ovalbumin. Lungs from each mouse were collected to determine NGF and related receptor expressions using real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. NGF and TrkA mRNAs were increased in the lungs of the immunized mice following exposure to 9 ppm toluene (n=6) (P<0.05 ppm vs. 0 ppm). Remarkably increased NGF-positive bronchiolus and alveolar epithelium cells were observed in 9 ppm toluene-exposed, immunized mice. To determine NGF mediating signaling, we also examined mRNA expression of neurotrophin receptor p75 (p75(NTR)) and oxidative stress marker, heme oxygenase (HO)-1 in the lung. There is no difference in the expressions of p75(NTR) and HO-1 between toluene-exposed and control mice. The expression of CCL2 and CCL3 mRNAs was significantly elevated in 9 ppm toluene-exposed, immunized mice. These findings suggest that the exposure with volatile organic compounds enhanced NGF expression and airway inflammation stronger in allergic individuals than in healthy individuals" |
| Keywords: | "Administration, Intranasal Animals Chemokine CCL2/biosynthesis/genetics Chemokine CCL4/biosynthesis/genetics Heme Oxygenase-1/biosynthesis/genetics Immunization Schedule Immunohistochemistry Lung/drug effects/*metabolism Male Mice Mice, Inbred C3H Nerve G;" |
| Notes: | "MedlineFujimaki, Hidekazu Tin-Tin-Win-Shwe Yamamoto, Shoji Nakajima, Daisuke Goto, Sumio eng Research Support, Non-U.S. Gov't Netherlands 2009/09/22 Toxicol Lett. 2009 Dec 15; 191(2-3):240-5. doi: 10.1016/j.toxlet.2009.09.004. Epub 2009 Sep 16" |
