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Talanta

Title:		Impact of the adsorbent material on volatile metabolites during in vitro and in vivo bio-sampling
Author(s):		Franchina FA; Zanella D; Dejong T; Focant JF;
Address:		"Molecular System, Organic & Biological Analytical Chemistry Group, University of Liege, Liege, Belgium. Electronic address: flaviofranchina@gmail.com. Molecular System, Organic & Biological Analytical Chemistry Group, University of Liege, Liege, Belgium"
Journal Title:		Talanta
Year:		2021
Volume:		20200827
Issue:
Page Number:		121569 -
DOI:		10.1016/j.talanta.2020.121569
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"The increased attraction of biological volatile compounds has opened the route to a wide variety of sampling techniques, amongst which trap tubes packed with adsorbent materials are commonly used. Many types of adsorbent materials are available and the choice of the adsorbent can impact the obtained results in untargeted analysis. Therefore, a proper combination of the adsorbent material and the sample is necessary to increase the robustness and reproducibility of biological studies. In this study, the sampling performance of thermal desorption tubes with six common adsorbent material combinations, i.e., Tenax(R) TA, Tenax(R) TA/Carbopack B, Tenax(R) TA/Sulficarb, Tenax(R) TA/Carbograph 5TD, Tenax(R) TA/Carbograph 1TD/Carboxen(R) 1003, and Carboxen(R) 1016/Carbograph 5TD, was evaluated in two different setups: in vitro and in vivo sampling. The in vitro setup consisted of the headspace dynamic extraction of spiked serum, and a mixture of 19 standards was evaluated in terms of response and reproducibility. The in vivo setup consisted into two parts: the first one was based the evaluation of the standard mixture, which was flash-vaporised into Tedlar(R) bags containing exhaled breath; the second part was based on the longitudinal monitoring of breath metabolites originating from a beverage intake (i.e., brewed coffee), over a 90?ª+min time period. The tubes were all desorbed and analysed in a comprehensive two-dimensional gas chromatography system coupled to a high-resolution time-of-flight mass spectrometer (GC?ª+x?ª+GC-HR ToF MS). In both sampling setups, the widest analytes coverage and the overall best extraction yield on the selected compounds were obtained using Tenax(R) TA, followed by Tenax(R) TA/Carbopack B. Tenax(R) TA provided the highest sampling reproducibility with 12 %RSD, 10 %RSD and <5 %RSD of the response during the experiments using the in vitro setup, the in vivo setup, and during the longitudinal tracking, respectively"
Keywords:		Exhalation Gas Chromatography-Mass Spectrometry Mass Spectrometry Reproducibility of Results *Volatile Organic Compounds/analysis Adsorbent tubes Comprehensive two-dimensional gas chromatography High-resolution mass spectrometry Metabolomics Thermal desor;
Notes:		"MedlineFranchina, Flavio A Zanella, Delphine Dejong, Thibaut Focant, Jean-Francois eng Netherlands 2020/11/11 Talanta. 2021 Jan 15; 222:121569. doi: 10.1016/j.talanta.2020.121569. Epub 2020 Aug 27"