| Title: | Clove Buds Volatile Compounds: Inhibitory Activity on Mycobacterium Growth and Molecular Docking on Mmr Efflux Pump Drug Resistance |
| Author(s): | El Ghallab Y; Eddine JJ; Aainouss A; El Messaoudi MD; Dakir M; Derfoufi S; |
| Address: | "Laboratory of Drugs Sciences, Biomedical Research and Biotechnology, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, B.P. 9154, Casablanca, 20250, Morocco. Laboratory of Organic Synthesis, Extraction and Valorization, Department of Chemistry, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, B.P. 5366, Casablanca, 20000, Morocco. Laboratory of Mycobacteria and Tuberculosis, Institut Pasteur of Morocco, 1 place Louis Pasteur, 20360, Casablanca, Morocco" |
| ISSN/ISBN: | 1612-1880 (Electronic) 1612-1872 (Linking) |
| Abstract: | "Syzygium aromaticum is used in traditional and modern medicine for its various and outstanding pharmacological properties. Here, we studied the chemical composition of hexane extract and non-polar fractions (NPF) obtained from the maceration and fractionation of clove buds, in order to evaluate their in vitro antimycobacterial activity, as well as their contribution against efflux pump (EP) resistance through molecular docking experiments. The gas chromatography-mass spectrometry (GC-MS) analysis of the volatile profiles revealed the presence of eugenol, followed by eugenyl acetate, and beta-caryophyllene as common major compounds. According to Resazurin microtiter assay (REMA), Mycobacterium tuberculosis H(37) Rv strain was sensitive to all volatile samples at concentration range between 10 and 100 mug/mL. The NPF of ethanol extract was the best inhibitor with a MIC=10 mug/mL. The in silico study revealed a strong binding affinity between eugenol and Mmr EP protein (-8.1 Kcal/mol), involving two binding modes of hydrogen bond and pi-alkyl interactions. The non-polarity character of clove volatile constituents, and their potential additive or synergistic effects could be responsible for the antimycobacterial activity. In addition, these findings suggest the benefic effect of eugenol in the management of mycobacterium drug resistance, whether as potential inhibitor of Mmr drug EP, or modulator during combination therapy" |
| Keywords: | antimycobacterial activity drug efflux pump natural products syzygium aromaticum terpenoids; |
| Notes: | "PublisherEl Ghallab, Yassine Eddine, Jamal Jamal Aainouss, Achraf El Messaoudi, My Driss Dakir, Mohamed Derfoufi, Sanae eng Faculty of Sciences Ain Chock/ Hassan II University of Casablanca/ Switzerland 2023/09/12 Chem Biodivers. 2023 Sep 11:e202300895. doi: 10.1002/cbdv.202300895" |
