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Curr Genet

Title:		Dse1 may control cross talk between the pheromone and filamentation pathways in yeast
Author(s):		Draper E; Dubrovskyi O; Bar EE; Stone DE;
Address:		"Laboratory for Molecular Biology (M/C 567), Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA"
Journal Title:		Curr Genet
Year:		2009
Volume:		20091010
Issue:		6
Page Number:		611 - 621
DOI:		10.1007/s00294-009-0274-6
ISSN/ISBN:		1432-0983 (Electronic) 0172-8083 (Linking)
Abstract:		"The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, they utilize many of the same signaling components. This implies the need for robust mechanisms to maintain signal fidelity. Dse1 was identified in an allele-specific suppressor screen for proteins that interact with the pheromone-responsive Gbetagamma, and found to bind both to a Gbetagamma-affinity column, and to the shared MEKK, Ste11. Although overexpression of Dse1 stimulated invasive growth and transcription of both filamentation and mating-specific transcriptional reporters, deletion of DSE1 had no effect on these outputs. In contrast, pheromone hyper-induced transcription of the filamentation reporter in cells lacking Dse1 and in cells expressing a mutant form of Gbeta that exhibits diminished interaction with Dse1. Thus, the interaction of Dse1 with both Gbeta and Ste11 may be designed to control cross talk between the pheromone and filamentation pathways"
Keywords:		"Binding Sites Carrier Proteins/genetics/*physiology Cell Cycle/drug effects Chromatography, Affinity GTP-Binding Protein beta Subunits/genetics/*physiology Gene Knockdown Techniques Genes, Reporter Haploidy MAP Kinase Kinase Kinases/genetics/*physiology M;"
Notes:		"MedlineDraper, Edward Dubrovskyi, Oleksii Bar, Eli E Stone, David E eng Comparative Study Research Support, U.S. Gov't, Non-P.H.S. 2009/10/13 Curr Genet. 2009 Dec; 55(6):611-21. doi: 10.1007/s00294-009-0274-6. Epub 2009 Oct 10"