| Title: | Current methods for detecting ethylene in plants |
| Author(s): | Cristescu SM; Mandon J; Arslanov D; De Pessemier J; Hermans C; Harren FJ; |
| Address: | "Department of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, NL 6525 AJ Nijmegen, The Netherlands. s.cristescu@science.ru.nl" |
| ISSN/ISBN: | 1095-8290 (Electronic) 0305-7364 (Print) 0305-7364 (Linking) |
| Abstract: | "BACKGROUND: In view of ethylene's critical developmental and physiological roles the gaseous hormone remains an active research topic for plant biologists. Progress has been made to understand the ethylene biosynthesis pathway and the mechanisms of perception and action. Still numerous questions need to be answered and findings to be validated. Monitoring gas production will very often complete the picture of any ethylene research topic. Therefore the search for suitable ethylene measuring methods for various plant samples either in the field, greenhouses, laboratories or storage facilities is strongly motivated. SCOPE: This review presents an update of the current methods for ethylene monitoring in plants. It focuses on the three most-used methods - gas chromatography detection, electrochemical sensing and optical detection - and compares them in terms of sensitivity, selectivity, time response and price. Guidelines are provided for proper selection and application of the described sensor methodologies and some specific applications are illustrated of laser-based detector for monitoring ethylene given off by Arabidopsis thaliana upon various nutritional treatments. CONCLUSIONS: Each method has its advantages and limitations. The choice for the suitable ethylene sensor needs careful consideration and is driven by the requirements for a specific application" |
| Keywords: | "Arabidopsis/*chemistry Biosensing Techniques/instrumentation/*methods Chromatography, Gas Electrochemical Techniques/instrumentation/*methods Ethylenes/*analysis/biosynthesis Lasers Plant Growth Regulators/*analysis Reproducibility of Results Sensitivity;" |
| Notes: | "MedlineCristescu, Simona M Mandon, Julien Arslanov, Denis De Pessemier, Jerome Hermans, Christian Harren, Frans J M eng Research Support, Non-U.S. Gov't Review England 2012/12/18 Ann Bot. 2013 Mar; 111(3):347-60. doi: 10.1093/aob/mcs259. Epub 2012 Dec 12" |
