| Title: | HS-SPME-GCxGC-qMS volatile metabolite profiling of Chrysolina herbacea frass and Mentha spp. leaves |
| Author(s): | Cordero C; Zebelo SA; Gnavi G; Griglione A; Bicchi C; Maffei ME; Rubiolo P; |
| Address: | "Dipartimento di Scienza e Tecnologia del Farmaco, Universita degli Studi di Torino, Via Pietro Giuria n degrees 9, 10125 Torino, Italy" |
| DOI: | 10.1007/s00216-011-5600-4 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "Headspace solid-phase microextraction (HS-SPME) comprehensive two-dimensional (2D) gas chromatography combined with quadrupole-mass spectrometry (GCxGC-qMS) with dedicated comparative data elaboration was applied to separate chemical patterns arising from the interaction between some Mentha species and the herbivore Chrysolina herbacea, also known as the mint bug. Upon feeding on different Mentha species (Mentha spicata L., Mentha x piperita L. and Mentha longifolia L.), C. herbacea produced frass (faeces) which were characterized by a typical volatile fraction. HS-SPME GCxGC-qMS analysis of the complex volatile fraction of both mint leaf and C. herbacea frass was submitted to advanced fingerprinting analysis of 2D chromatographic data. 1,8-Cineole, found in the leaves of all the Mentha species examined, was oxidized, and C. herbacea frass yielded high rates of several hydroxy-1,8-cineoles, including 2alpha-hydroxy-, 3alpha-hydroxy-, 3beta-hydroxy- and 9-hydroxy-1,8-cineole. Upon insect feeding, several unknown oxidized monoterpenes, a p-menthane diol and three unknown phenylpropanoids were also detected in the frass volatiles. In M. longifolia, the occurrence of the monoterpene piperitenone oxide was found to be toxic and associated with insect death. The results of this work show that high throughput techniques such as HS-SPME and GCxGC-qMS fingerprint analysis are ideal tools to analyze complex volatile matrices, and provide a sensitive method for the direct comparison and chemical visualization of plant and insect emitted volatile components" |
| Keywords: | "Animals Coleoptera/*physiology Cyclohexanols/analysis Eucalyptol Gas Chromatography-Mass Spectrometry/*methods *Herbivory Mentha/*chemistry/physiology Monoterpenes/analysis Oils, Volatile/analysis Plant Leaves/*chemistry Sensitivity and Specificity Solid;" |
| Notes: | "MedlineCordero, Chiara Zebelo, Simon Atsbaha Gnavi, Giorgio Griglione, Alessandra Bicchi, Carlo Maffei, Massimo E Rubiolo, Patrizia eng Research Support, Non-U.S. Gov't Germany 2011/12/07 Anal Bioanal Chem. 2012 Feb; 402(5):1941-52. doi: 10.1007/s00216-011-5600-4. Epub 2011 Dec 6" |
