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J Econ Entomol

Title:		A Real-Time PCR Assay for Rapid Identification of Tuta absoluta (Lepidoptera: Gelechiidae)
Author(s):		Zink FA; Tembrock LR; Timm AE; Gilligan TM;
Address:		"Department of Agricultural Biology, 1177 Campus Delivery, Colorado State University, Fort Collins, CO. USDA-APHIS-PPQ-Science & Technology, Identification Technology Program, Fort Collins, CO"
Journal Title:		J Econ Entomol
Year:		2020
Volume:		113
Issue:		3
Page Number:		1479 - 1485
DOI:		10.1093/jee/toaa040
ISSN/ISBN:		1938-291X (Electronic) 0022-0493 (Linking)
Abstract:		"The tomato leafminer, Tuta absoluta (Meyrick), is a highly destructive pest of tomatoes, causing damage to leaves, stalks, buds, and fruits. Native to South America, T. absoluta is now found throughout Europe, South Asia, Africa, parts of Central America, and the Caribbean. Adults are small, with a wingspan of approximately one cm and lack distinctive markings, making morphological identification difficult. Larvae are also difficult to identify and resemble those of many other gelechiids. Due to the extensive time spent and expertise required for morphological identification, and the imminent threat to the North American tomato crop, we have developed a rapid molecular test for discriminating individual specimens of T. absoluta using a probe-based real-time polymerase chain reaction (PCR) assay. The assay is able to quickly distinguish T. absoluta from similar-sized moth specimens that are attracted to T. absoluta pheromone lures in the United States and is also able to identify larvae of T. absoluta. Decreased identification time for this critical pest will lead to more rapid identification at ports of entry and allow for more efficient trap screening for domestic monitoring programs"
Keywords:		Africa Animals Asia Central America Europe *Lepidoptera *Solanum lycopersicum *Moths/genetics Real-Time Polymerase Chain Reaction South America crop pest droplet digital PCR invasive species real-time PCR tomato leafminer;
Notes:		"MedlineZink, Frida A Tembrock, Luke R Timm, Alicia E Gilligan, Todd M eng Research Support, U.S. Gov't, Non-P.H.S. England 2020/03/17 J Econ Entomol. 2020 Jun 6; 113(3):1479-1485. doi: 10.1093/jee/toaa040"