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J Agric Food Chem

Title:		Rapid diagnosis of the infection of pine tree with pine wood nematode (Bursaphelenchus xylophilus) by use of host-tree volatiles
Author(s):		Yun JE; Kim J; Park CG;
Address:		"Institute of Agriculture and Life Sciences, and double daggerDivision of Applied Life Science (BK21 Program), Gyeongsang National University , Jinju, Gyeongnam 660-701, Republic of Korea"
Journal Title:		J Agric Food Chem
Year:		2012
Volume:		20120717
Issue:		30
Page Number:		7392 - 7397
DOI:		10.1021/jf302484m
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Attraction of the Bursaphelenchus xylophilus nematode toward 18 volatiles of Pinus species was evaluated by a Petri-dish bioassay under laboratory conditions to develop a rapid diagnostic kit. Among these compounds, alpha-pinene, beta-pinene, and camphor showed significantly higher attractiveness to B. xylophilus in both the reproductive and dispersal stages, whereas these compounds were not active against Bursaphelenchus mucronatus . A trap tube was developed as a diagnostic kit, which consisted of a tube filled with 0.8% agar and a matrix impregnated with an attractant: alpha-pinene, beta-pinene, or camphor. All tested compounds attracted a significantly higher number of B. xylophilus than that in the control treatment. No significant difference was observed among attractants. The cotton-ball matrix was significantly more effective than the filter-paper matrix for attracting B. xylophilus in the artificial pupal chamber bioassay. In a bioassay with pine wood nematode (PWN)-infected pine tree logs, B. xylophilus was initially attracted after an 8 h trap period and the number of B. xylophilus increased with time. The trap tube using camphor and the cotton-ball matrix were most effective for attracting B. xylophilus . The semiochemical-based tube-trapping method is simple to use, requires minimal labor, and is economical and effective for detecting B. xylophilus living in host pine trees during field sampling"
Keywords:		Animals Bicyclic Monoterpenes Bridged Bicyclo Compounds/analysis Camphor/analysis Host-Parasite Interactions/*physiology Monoterpenes/analysis Pinus/chemistry/*parasitology Plant Diseases/*parasitology Tylenchida/*growth & development Volatile Organic Com;
Notes:		"MedlineYun, Ji Eun Kim, Junheon Park, Chung Gyoo eng 2012/06/26 J Agric Food Chem. 2012 Aug 1; 60(30):7392-7. doi: 10.1021/jf302484m. Epub 2012 Jul 17"