| Title: | Sciadopitys verticillata Resin: Volatile Components and Impact on Plant Pathogenic and Foodborne Bacteria |
| Author(s): | Yates DI; Ownley BH; Labbe N; Bozell JJ; Klingeman WE; Batson EK; Gwinn KD; |
| Address: | "Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 210095, USA. yatesd@wcde.org. Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 210095, USA. bownley@utk.edu. Center for Renewable Carbon, University of Tennessee, Knoxville, TN 210095, USA. nlabbe@utk.edu. Center for Renewable Carbon, University of Tennessee, Knoxville, TN 210095, USA. jbozell@utk.edu. Plant Sciences, University of Tennessee, Knoxville, TN 210095, USA. wklingem@utk.edu. Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 210095, USA. emmabat@MIT.EDU. Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 210095, USA. kgwinn@utk.edu" |
| DOI: | 10.3390/molecules24203767 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Sciadopitys verticillata (Sv) produces a white, sticky, latex-like resin with antimicrobial properties. The aims of this research were to evaluate the effects of this resin (Sv resin) on bacterial populations and to determine the impact of its primary volatile components on bioactivity. The impact of sample treatment on chemical composition of Sv resin was analyzed using Fourier transform infrared spectroscopy (FTIR) coupled with principal component analysis. The presence and concentration of volatiles in lyophilized resin were determined using gas chromatography/mass spectrometry (GC/MS). Changes in bacterial population counts due to treatment with resin or its primary volatile components were monitored. Autoclaving of the samples did not affect the FTIR spectra of Sv resin; however, lyophilization altered spectra, mainly in the CH and C=O regions. Three primary bioactive compounds that constituted >90% of volatiles (1R-alpha-pinene, tricyclene, and beta-pinene) were identified in Sv resin. Autoclaved resin impacted bacterial growth. The resin was stimulatory for some plant and foodborne pathogens (Pseudomonas fluorescens, P. syringae, and Xanthomonas perforans) and antimicrobial for others (Escherichia coli, Bacillus cereus, Agrobacterium tumefaciens, and Erwinia amylovora). Treatment with either 1R-alpha-pinene or beta-pinene reduced B. cereus population growth less than did autoclaved resin. The complex resin likely contains additional antimicrobial compounds that act synergistically to inhibit bacterial growth" |
| Keywords: | "Anti-Infective Agents/chemistry/*pharmacology Bacteria/drug effects/growth & development Drug Synergism Food Microbiology Gas Chromatography-Mass Spectrometry Plant Pathology Principal Component Analysis Resins, Plant/*chemistry Spectroscopy, Fourier Tran;" |
| Notes: | "MedlineYates, David I Ownley, Bonnie H Labbe, Nicole Bozell, Joseph J Klingeman, William E Batson, Emma K Gwinn, Kimberly D eng Switzerland 2019/10/23 Molecules. 2019 Oct 19; 24(20):3767. doi: 10.3390/molecules24203767" |
