| Title: | Symbiosis induces unique volatile profiles in the model cnidarian Aiptasia |
| Author(s): | Wuerz M; Lawson CA; Ueland M; Oakley CA; Grossman AR; Weis VM; Suggett DJ; Davy SK; |
| Address: | "School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand. Climate Change Cluster, University of Technology Sydney, Sydney Broadway, NSW 2007, Australia. School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia. Centre for Forensic Science, School of Mathematical and Physical Sciences, University of Technology Sydney, Sydney Broadway, NSW 2007, Australia. Carnegie Institution of Washington, Department of Plant Biology, Stanford, CA 94305, USA. Department of Integrative Biology, Oregon State University, Corvallis, OR 97331, USA" |
| ISSN/ISBN: | 1477-9145 (Electronic) 0022-0949 (Linking) |
| Abstract: | "The establishment and maintenance of the symbiosis between a cnidarian host and its dinoflagellate symbionts is central to the success of coral reefs. To explore the metabolite production underlying this symbiosis, we focused on a group of low molecular weight secondary metabolites, biogenic volatile organic compounds (BVOCs). BVOCs are released from an organism or environment, and can be collected in the gas phase, allowing non-invasive analysis of an organism's metabolism (i.e. 'volatilomics'). We characterised volatile profiles of the sea anemone Aiptasia (Exaiptasia diaphana), a model system for cnidarian-dinoflagellate symbiosis, using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry. We compared volatile profiles between: (1) symbiotic anemones containing their native symbiont, Breviolum minutum; (2) aposymbiotic anemones; and (3) cultured isolates of B. minutum. Overall, 152 BVOCs were detected, and classified into 14 groups based on their chemical structure, the most numerous groups being alkanes and aromatic compounds. A total of 53 BVOCs were differentially abundant between aposymbiotic anemones and B. minutum cultures; 13 between aposymbiotic and symbiotic anemones; and 60 between symbiotic anemones and cultures of B. minutum. More BVOCs were differentially abundant between cultured and symbiotic dinoflagellates than between aposymbiotic and symbiotic anemones, suggesting that symbiosis may modify symbiont physiology more than host physiology. This is the first volatilome analysis of the Aiptasia model system and provides a foundation from which to explore how BVOC production is perturbed under environmental stress, and ultimately the role they play in this important symbiosis" |
| Keywords: | Alkanes Animals *Dinoflagellida/physiology *Sea Anemones/physiology Symbiosis *Volatile Organic Compounds BVOCs Cell signalling Homologous Symbiodiniaceae Volatilomics; |
| Notes: | "MedlineWuerz, Maggie Lawson, Caitlin A Ueland, Maiken Oakley, Clinton A Grossman, Arthur R Weis, Virginia M Suggett, David J Davy, Simon K eng Victoria University/ JEBTF1908268/Company of Biologists/ 19-VUW-086 489/Royal Society Te Aprangi/ DP200100091/ARC Discovery Project/ 19-VUW-086/Royal Society of New Zealand/ DP200100091/Australian Research Council discovery project/ Research Support, Non-U.S. Gov't England 2022/09/27 J Exp Biol. 2022 Oct 1; 225(19):jeb244600. doi: 10.1242/jeb.244600. Epub 2022 Oct 12" |
