| Title: | Use of headspace SPME-GC-MS for the analysis of the volatiles produced by indoor molds grown on different substrates |
| Author(s): | Van Lancker F; Adams A; Delmulle B; De Saeger S; Moretti A; Van Peteghem C; De Kimpe N; |
| Address: | "Department of Organic Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium" |
| ISSN/ISBN: | 1464-0325 (Print) 1464-0325 (Linking) |
| Abstract: | "An automated headspace solid phase microextraction method followed by GC-MS analysis was used to evaluate and compare the in vitro production of microbial volatile organic compounds (MVOCs) on malt extract agar, plasterboard and wallpaper. Five fungal strains were isolated from the walls of water-damaged houses and identified. In addition, four other common molds were studied. In general, MVOC production was the highest on malt extract agar. On this synthetic medium, molds typically produced 2-methylpropanol, 2-methylbutanol and 3-methylbutanol. On wallpaper, mainly 2-ethylhexanol, methyl 2-ethylhexanoate and compounds of the C8-complex such as 1-octene-3-ol, 3-octanone, 3-octanol and 1,3-octadiene were detected. The detection of 2-ethylhexanol and methyl 2-ethylhexanoate indicates an enhanced degradation of the substrate by most fungi. For growth on plasterboard, no typical metabolites were detected. Despite these metabolite differences on malt extract agar, wallpaper and plasterboard, some molds also produced specific compounds independently of the used substrate, such as trichodiene from Fusarium sporotrichioides and aristolochene from Penicillium roqueforti. Therefore, these metabolites can be used as markers for the identification and maybe also mycotoxin production of these molds. All five investigated Penicillium spp. in this study were able to produce two specific diterpenes, which were not produced by the other species studied. These two compounds, which remain unidentified until now, therefore seem specific for Penicillium spp. and are potentially interesting for the monitoring of this fungal genus. Further experiments will be performed with other Penicillium spp. to study the possibility that these two compounds are specific for this group of molds" |
| Keywords: | "Air Pollutants/*analysis/chemistry/metabolism Air Pollution, Indoor/*analysis Alternaria/chemistry/classification/metabolism Aspergillus/chemistry/classification/metabolism Construction Materials *Environmental Monitoring Fungi/*chemistry/classification/m;" |
| Notes: | "MedlineVan Lancker, Fien Adams, An Delmulle, Barbara De Saeger, Sarah Moretti, Antonio Van Peteghem, Carlos De Kimpe, Norbert eng Research Support, Non-U.S. Gov't England 2008/10/10 J Environ Monit. 2008 Oct; 10(10):1127-33. doi: 10.1039/b808608g" |
