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« Previous AbstractMonoterpenyl esters in juvenile mountain pine beetle and sex-specific release of the aggregation pheromone trans-verbenol    Next AbstractCytochromes P450 Preferentially Expressed in Antennae of the Mountain Pine Beetle »

Sci Rep


Title:The cytochrome P450 CYP6DE1 catalyzes the conversion of alpha-pinene into the mountain pine beetle aggregation pheromone trans-verbenol
Author(s):Chiu CC; Keeling CI; Bohlmann J;
Address:"Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, B.C., V6T 1Z4, Canada. Department of Botany, University of British Columbia, 6270 University Blvd, Vancouver, B.C., V6T 1Z4, Canada. Laurentian Forestry Centre, Canadian Forest Service, Natural Resources Canada, 1055 rue du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy, Quebec, QC, G1V 4C7, Canada. Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, B.C., V6T 1Z4, Canada. bohlmann@msl.ubc.ca. Department of Botany, University of British Columbia, 6270 University Blvd, Vancouver, B.C., V6T 1Z4, Canada. bohlmann@msl.ubc.ca"
Journal Title:Sci Rep
Year:2019
Volume:20190206
Issue:1
Page Number:1477 -
DOI: 10.1038/s41598-018-38047-8
ISSN/ISBN:2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:"The recent outbreak of the mountain pine beetle (Dendroctonus ponderosae; MPB) has affected over 20 M hectares of pine forests in western North America. During the colonization of host trees, female MPB release the aggregation pheromone (-)-trans-verbenol. (-)-trans-Verbenol is thought to be produced from the pine defense compound (-)-alpha-pinene by cytochrome P450 (P450) dependent hydroxylation. MPB may also use P450s for the detoxification of other monoterpenes of the pine defense system. Here we describe the functional characterization of MPB CYP6DE1. CYP6DE1, but not the closely related CYP6DE2, used the bicyclic monoterpenes (-)-alpha-pinene, (+)-alpha-pinene, (-)-beta-pinene, (+)-beta-pinene and (+)-3-carene as substrates. CYP6DE1 was not active with other monoterpenes or diterpene resin acids that were tested as substrates. trans-Verbenol is the major product of CYP6DE1 activity with (-)-alpha-pinene or (+)-alpha-pinene as substrates. When tested with blends of different ratios of (-)-alpha-pinene and (+)-alpha-pinene, CYP6DE1 produced trans-verbenol with an enantiomeric profile that was similar to that produced by female MPB exposed to the alpha-pinene enantiomers"
Keywords:Animals Bicyclic Monoterpenes/*metabolism Coleoptera Cytochrome P-450 Enzyme System/genetics/*metabolism Female Hydroxylation Insect Proteins/genetics/metabolism Sf9 Cells;
Notes:"MedlineChiu, Christine C Keeling, Christopher I Bohlmann, Joerg eng Research Support, Non-U.S. Gov't England 2019/02/08 Sci Rep. 2019 Feb 6; 9(1):1477. doi: 10.1038/s41598-018-38047-8"

 
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Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
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