| Title: | "Transgenic cry1C(?n-) gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae)" |
| Author(s): | Sun X; Yan MJ; Zhang A; Wang MQ; |
| Address: | "Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Invasive Insect Biocontrol and Behavior Laboratory, BARC-West, USDA-ARS, Beltsville, MD 20705-2350, United States. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: mqwang@mail.hzau.edu.cn" |
| DOI: | 10.1016/j.ecoenv.2015.06.034 |
| ISSN/ISBN: | 1090-2414 (Electronic) 0147-6513 (Linking) |
| Abstract: | "Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatiles cues, physical characteristics, and taste chemicals on the grains could be the important key behavior factors for storage insect pests to locate the hosts and select oviposition sites. It is also well known that the transgenic Bt rough rice line T1C-19, which expresses a cry1C(?n-) gene has a high resistance to Lepidoptera pests. However, there were no evidences to show the consequences of host preference for non-target insect pests after growing Bt transgenic rice. In this study, the potential key factors of Bt rough rice were investigated for their impacts on the behaviors of non-target pest lesser grain borer Rhyzopertha dominica, the main weevil pest of grain and its parasitic wasps Anisopteromalus calandrae, the natural enemy of the beetle. Both electronic nose and electronic tongue analyses showed that the parameters of Bt rough rice were analogous to those of the non-Bt rough rice. The volatile profiles of Bt and non-Bt rough rice examined by gas chromatographic mass spectrometry (GC-MS) were similar. For most volatile compounds, there were no significantly quantitative differences in compound quantities between Bt and non-Bt rough rice. The densities of sclereids and trichomes on the rough rice husk surface were statistically equal in Bt and non-Bt rough rice. The non-target pest, R. dominica, and its parasitoid wasp, A. calandrae, were attracted to both rough rice and could not distinguish the transgenic T1C-19 from the isogenic rough rice. These results demonstrated that Bt rough rice has no negative impacts on the host preference behaviors of non-target stored product pest R. dominica and its parasitoid A. calandrae" |
| Keywords: | "Animals Biological Assay Coleoptera/*metabolism Food Contamination/analysis Food Parasitology Food Storage Gas Chromatography-Mass Spectrometry *Gene Expression Regulation, Plant Olfactometry Oryza/*genetics/*parasitology Oviposition/physiology Pest Contr;" |
| Notes: | "MedlineSun, Xiao Yan, Miao-Jun Zhang, Aijun Wang, Man-Qun eng Research Support, Non-U.S. Gov't Netherlands 2015/07/08 Ecotoxicol Environ Saf. 2015 Oct; 120:449-56. doi: 10.1016/j.ecoenv.2015.06.034. Epub 2015 Jul 3" |
