| Title: | OXI1 kinase plays a key role in resistance of Arabidopsis towards aphids (Myzus persicae) |
| Author(s): | Shoala T; Edwards MG; Knight MR; Gatehouse AMR; |
| Address: | "School of Natural and Environmental Sciences, Newcastle University, Newcastle-Upon-Tyne, NE1 7RU, UK. College of Biotechnology, Misr University for Science and Technology, Giza, Egypt. School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE, UK. School of Natural and Environmental Sciences, Newcastle University, Newcastle-Upon-Tyne, NE1 7RU, UK. a.m.r.gatehouse@ncl.ac.uk" |
| DOI: | 10.1007/s11248-018-0078-x |
| ISSN/ISBN: | 1573-9368 (Electronic) 0962-8819 (Linking) |
| Abstract: | "Plants have co-evolved with a diverse array of pathogens and insect herbivores and so have evolved an extensive repertoire of constitutive and induced defence mechanisms activated through complex signalling pathways. OXI1 kinase is required for activation of mitogen-activated protein kinases (MAPKs) and is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses. Furthermore, changes in the levels of OXI1 appear to be crucial for appropriate signalling. Callose deposition also plays a key role in defence. Here we demonstrate, for the first time, that OXI1 plays an important role in defence against aphids. The Arabidopsis mutant, oxi1-2, showed significant resistance both in terms of population build-up (p < 0.001) and the rate of build-up (p < 0.001). Arabidopsis mutants for beta-1,3-glucanase, gns2 and gns3, showed partial aphid resistance, significantly delaying developmental rate, taking two-fold longer to reach adulthood. Whilst beta-1,3-glucanase genes GNS1, GNS2, GNS3 and GNS5 were not induced in oxi1-2 in response to aphid feeding, GNS2 was expressed to high levels in the corresponding WT (Col-0) in response to aphid feeding. Callose synthase GSL5 was up-regulated in oxi1-2 in response to aphids. The results suggest that resistance in oxi1-2 mutants is through induction of callose deposition via MAPKs resulting in ROS induction as an early response. Furthermore, the results suggest that the beta-1,3-glucanase genes, especially GNS2, play an important role in host plant susceptibility to aphids. Better understanding of signalling cascades underpinning tolerance to biotic stress will help inform future breeding programmes for enhancing crop resilience" |
| Keywords: | "Animals Aphids/genetics/pathogenicity Arabidopsis/genetics/growth & development/*parasitology Arabidopsis Proteins/*genetics Disease Resistance/*genetics Drug Tolerance Gene Expression Regulation, Plant/genetics Plant Breeding Plant Diseases/*genetics/par;" |
| Notes: | "MedlineShoala, Tahsin Edwards, Martin G Knight, Marc R Gatehouse, Angharad M R eng Netherlands 2018/05/20 Transgenic Res. 2018 Aug; 27(4):355-366. doi: 10.1007/s11248-018-0078-x. Epub 2018 May 17" |
