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Eukaryot Cell

Title:		The Ras/protein kinase A pathway acts in parallel with the Mob2/Cbk1 pathway to effect cell cycle progression and proper bud site selection
Author(s):		Schneper L; Krauss A; Miyamoto R; Fang S; Broach JR;
Address:		"Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA"
Journal Title:		Eukaryot Cell
Year:		2004
Volume:		3
Issue:		1
Page Number:		108 - 120
DOI:		10.1128/EC.3.1.108-120.2004
ISSN/ISBN:		1535-9778 (Print) 1535-9786 (Electronic) 1535-9786 (Linking)
Abstract:		"In Saccharomyces cerevisiae, Ras proteins connect nutrient availability to cell growth through regulation of protein kinase A (PKA) activity. Ras proteins also have PKA-independent functions in mitosis and actin repolarization. We have found that mutations in MOB2 or CBK1 confer a slow-growth phenotype in a ras2Delta background. The slow-growth phenotype of mob2Delta ras2Delta cells results from a G1 delay that is accompanied by an increase in size, suggesting a G1/S role for Ras not previously described. In addition, mob2Delta strains have imprecise bud site selection, a defect exacerbated by deletion of RAS2. Mob2 and Cbk1 act to properly localize Ace2, a transcription factor that directs daughter cell-specific transcription of several genes. The growth and budding phenotypes of the double-deletion strains are Ace2 independent but are suppressed by overexpression of the PKA catalytic subunit, Tpk1. From these observations, we conclude that the PKA pathway and Mob2/Cbk1 act in parallel to determine bud site selection and promote cell cycle progression"
Keywords:		"Actins/metabolism Blotting, Northern Cell Cycle Cell Cycle Proteins/*metabolism Cell Division Cell Survival Cyclic AMP-Dependent Protein Kinases/*metabolism Diploidy Flow Cytometry Fungal Proteins/*metabolism G1 Phase Gene Deletion Genotype Haploidy Intra;"
Notes:		"MedlineSchneper, Lisa Krauss, Alicia Miyamoto, Ryan Fang, Shirley Broach, James R eng F32 GM019308/GM/NIGMS NIH HHS/ P01 CA041086/CA/NCI NIH HHS/ 5F32 GM19308/GM/NIGMS NIH HHS/ CA41086/CA/NCI NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2004/02/12 Eukaryot Cell. 2004 Feb; 3(1):108-20. doi: 10.1128/EC.3.1.108-120.2004"