| Title: | Ultrafast gas chromatography coupled to electronic nose to identify volatile biomarkers in exhaled breath from chronic obstructive pulmonary disease patients: A pilot study |
| Author(s): | Rodriguez-Aguilar M; Ramirez-Garcia S; Ilizaliturri-Hernandez C; Gomez-Gomez A; Van-Brussel E; Diaz-Barriga F; Medellin-Garibay S; Flores-Ramirez R; |
| Address: | "Centro de Investigacion Aplicada en Ambiente y Salud, CIACYT, Facultad de Medicina, Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico. Pulmonology Service, Hospital Central 'Dr. Ignacio Morones Prieto' San Luis Potosi, San Luis Potosi, Mexico. Coordinacion para la Innovacion y Aplicacion de la Ciencia y la Tecnologia (CIACYT), Universidad Autonoma de San Luis Potosi, San Luis Potosi, S.L.P., Mexico" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "An analytical method to identify volatile organic compounds (VOCs) in the exhaled breath from patients with a diagnosis of chronic obstructive pulmonary disease (COPD) using a ultrafast gas chromatography system equipped with an electronic nose detector (FGC eNose) has been developed. A prospective study was performed in 23 COPD patients and 33 healthy volunteers; exhalation breathing tests were performed with Tedlar bags. Each sample was analyzed by FCG eNose and the identification of VOCs was based on the Kovats index. Raw data were reduced by principal component analysis (PCA) and canonical discriminant analysis [canonical analysis of principal coordinates (CAP)]. The FCG eNose technology was able to identify 17 VOCs that distinguish COPD patients from healthy volunteers. At all stages of PCA and CAP the discrimination between groups was obvious. Chemical prints were correctly classified up to 82.2%, and were matched with 78.9% of the VOCs detected in the exhaled breath samples. Receiver operating characteristic curve analysis indicated the sensitivity and specificity to be 96% and 91%, respectively. This pilot study demonstrates that FGC eNose is a useful tool to identify VOCs as biomarkers in exhaled breath from COPD patients. Further studies should be performed to enhance the clinical relevance of this quick and ease methodology for COPD diagnosis" |
| Keywords: | "Aged Biomarkers/analysis Breath Tests/*methods Chromatography, Gas/*methods *Electronic Nose Female Humans Male Middle Aged Pilot Projects *Pulmonary Disease, Chronic Obstructive/diagnosis/metabolism ROC Curve Reproducibility of Results Volatile Organic C;" |
| Notes: | "MedlineRodriguez-Aguilar, Maribel Ramirez-Garcia, Sofia Ilizaliturri-Hernandez, Cesar Gomez-Gomez, Alejandro Van-Brussel, Evelyn Diaz-Barriga, Fernando Medellin-Garibay, Susanna Flores-Ramirez, Rogelio eng SS/IMSS/ISSSTE-CONACYT #234111/National Council for Science and Technology/ 2015-1340/National Council for Science and Technology-National Problems/ England 2019/08/20 Biomed Chromatogr. 2019 Dec; 33(12):e4684. doi: 10.1002/bmc.4684. Epub 2019 Sep 9" |
