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Metabolomics

Title:		Volatile metabolomic signature of bladder cancer cell lines based on gas chromatography-mass spectrometry
Author(s):		Rodrigues D; Pinto J; Araujo AM; Monteiro-Reis S; Jeronimo C; Henrique R; de Lourdes Bastos M; de Pinho PG; Carvalho M;
Address:		"UCIBIO/REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal. daniela.fgrodrigues@gmail.com. UCIBIO/REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal. Cancer Biology & Epigenetics Group, Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal. Department of Pathology and Molecular Immunology-Biomedical Sciences Institute (ICBAS), University of Porto, Porto, Portugal. Department of Pathology, Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal. UCIBIO/REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal. mcarv@ufp.edu.pt. UFP Energy, Environment and Health Research Unit (FP-ENAS), University Fernando Pessoa, Porto, Portugal. mcarv@ufp.edu.pt"
Journal Title:		Metabolomics
Year:		2018
Volume:		20180417
Issue:		5
Page Number:		62 -
DOI:		10.1007/s11306-018-1361-9
ISSN/ISBN:		1573-3890 (Electronic) 1573-3882 (Linking)
Abstract:		"INTRODUCTION: Recent studies provide a convincing support that the presence of cancer cells in the body leads to the alteration of volatile organic compounds (VOCs) emanating from biological samples, particularly of those closely related with tumoral tissues. Thus, a great interest emerged for the study of cancer volatilome and subsequent attempts to confirm VOCs as potential diagnostic biomarkers. OBJECTIVES: The aim of this study was to determine the volatile metabolomic signature of bladder cancer (BC) cell lines and provide an in vitro proof-of-principle that VOCs emanated into the extracellular medium may discriminate BC cells from normal bladder epithelial cells. METHODS: VOCs in the culture media of three BC cell lines (Scaber, J82, 5637) and one normal bladder cell line (SV-HUC-1) were extracted by headspace-solid phase microextraction and analysed by gas chromatography-mass spectrometry (HS-SPME/GC-MS). Two different pH (pH 2 and 7) were used for VOCs extraction to infer the best pH to be used in in vitro metabolomic studies. RESULTS: Multivariate analysis revealed a panel of volatile metabolites that discriminated cancerous from normal bladder cells, at both pHs, although a higher number of discriminative VOCs was obtained at neutral pH. Most of the altered metabolites were ketones and alkanes, which were generally increased in BC compared to normal cells, and alcohols, which were significantly decreased in BC cells. Among them, three metabolites, namely 2-pentadecanone, dodecanal and gamma-dodecalactone (the latter only tentatively identified), stood out as particularly important metabolites and promising volatile biomarkers for BC detection. Furthermore, our results also showed the potential of VOCs in discriminating BC cell lines according to tumour grade and histological subtype. CONCLUSIONS: We demonstrate that a GC-MS metabolomics-based approach for analysis of VOCs is a valuable strategy for identifying new and specific biomarkers that may improve BC diagnosis. Future studies should entail the validation of volatile signature found for BC cell lines in biofluids from BC patients"
Keywords:		"Biomarkers Cell Line, Tumor Gas Chromatography-Mass Spectrometry/methods Humans Metabolomics/*methods Multivariate Analysis Solid Phase Microextraction/methods Urinary Bladder Neoplasms/chemistry/*metabolism Volatile Organic Compounds/analysis/*chemistry;"
Notes:		"MedlineRodrigues, Daniela Pinto, Joana Araujo, Ana Margarida Monteiro-Reis, Sara Jeronimo, Carmen Henrique, Rui de Lourdes Bastos, Maria de Pinho, Paula Guedes Carvalho, Marcia eng Research Support, Non-U.S. Gov't 2019/03/05 Metabolomics. 2018 Apr 17; 14(5):62. doi: 10.1007/s11306-018-1361-9"