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Int J Mol Sci

Title:		Transcriptome Dynamics Underlying Planticine((R))-Induced Defense Responses of Tomato (Solanum lycopersicum L.) to Biotic Stresses
Author(s):		Rakoczy-Lelek R; Czernicka M; Ptaszek M; Jarecka-Boncela A; Furmanczyk EM; Keska-Izworska K; Grzanka M; Skoczylas L; Kuznik N; Smolen S; Macko-Podgorni A; Gaska K; Chalanska A; Ambroziak K; Kardasz H;
Address:		"INTERMAG Sp. z o.o., Al. 1000-lecia 15G, 32-300 Olkusz, Poland. Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland. Department of Plant Protection, The National Institute of Horticultural Research, Konstytucji 3 Maja 1/3 Str., 96-100 Skierniewice, Poland. Department of Plant Product Technology and Nutrition Hygiene, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122 Str., 30-149 Krakow, Poland. Faculty of Chemistry, Silesian University of Technology, Krzywoustego 4 Str., 44-100 Gliwice, Poland. Laboratory of Mass Spectrometry, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland. NEFSCIENCE, Bohaterow Westerplatte 119 Str., 96-100 Skierniewice, Poland"
Journal Title:		Int J Mol Sci
Year:		2023
Volume:		20230330
Issue:		7
Page Number:		-
DOI:		10.3390/ijms24076494
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"The induction of natural defense mechanisms in plants is considered to be one of the most important strategies used in integrated pest management (IPM). Plant immune inducers could reduce the use of chemicals for plant protection and their harmful impacts on the environment. Planticine((R)) is a natural plant defense biostimulant based on oligomers of alpha(1-->4)-linked D-galacturonic acids, which are biodegradable and nontoxic. The aim of this study was to define the molecular basis of Planticine's biological activity and the efficacy of its use as a natural plant resistance inducer in greenhouse conditions. Three independent experiments with foliar application of Planticine((R)) were carried out. The first experiment in a climatic chamber (control environment, no pest pressure) subjected the leaves to RNA-seq analysis, and the second and third experiments in greenhouse conditions focused on efficacy after a pest infestation. The result was the RNA sequencing of six transcriptome libraries of tomatoes treated with Planticine((R)) and untreated plants; a total of 3089 genes were found to be differentially expressed genes (DEGs); among them, 1760 and 1329 were up-regulated and down-regulated, respectively. DEG analysis indicated its involvement in such metabolic pathways and processes as plant-pathogen interaction, plant hormone signal transduction, MAPK signaling pathway, photosynthesis, and regulation of transcription. We detected up-regulated gene-encoded elicitor and effector recognition receptors (ELRR and ERR), mitogen-activated protein kinase (MAPKs) genes, and transcription factors (TFs), i.e., WRKY, ERF, MYB, NAC, bZIP, pathogenesis-related proteins (PRPs), and resistance-related metabolite (RRMs) genes. In the greenhouse trials, foliar application of Planticine((R)) proved to be effective in reducing the infestation of tomato leaves by the biotrophic pathogen powdery mildew and in reducing feeding by thrips, which are insect herbivores. Prophylactic and intervention use of Planticine((R)) at low infestation levels allows the activation of plant defense mechanisms"
Keywords:		"*Transcriptome *Solanum lycopersicum/genetics Sequence Analysis, RNA Plant Growth Regulators/pharmacology Stress, Physiological Gene Expression Regulation, Plant Gene Expression Profiling Plant Diseases/genetics RNA-seq elicitor oligogalacturonides phytoh;"
Notes:		"MedlineRakoczy-Lelek, Roksana Czernicka, Malgorzata Ptaszek, Magdalena Jarecka-Boncela, Anna Furmanczyk, Ewa M Keska-Izworska, Kinga Grzanka, Marlena Skoczylas, Lukasz Kuznik, Nikodem Smolen, Sylwester Macko-Podgorni, Alicja Gaska, Klaudia Chalanska, Aneta Ambroziak, Krzysztof Kardasz, Hubert eng POIR.01.01.01-00-0024/15/European Union, European Regional Development Fund under Smart Growth Operational Program 2014-2020., National Center for Research and Development: Szybka Sciezka/ Switzerland 2023/04/14 Int J Mol Sci. 2023 Mar 30; 24(7):6494. doi: 10.3390/ijms24076494"