| Title: | Transcriptome analysis of aphids exposed to glandular trichomes in tomato reveals stress and starvation related responses |
| Author(s): | Planello R; Llorente L; Herrero O; Novo M; Blanco-Sanchez L; Diaz-Pendon JA; Fernandez-Munoz R; Ferrero V; de la Pena E; |
| Address: | "Biology and Environmental Toxicology Group, Faculty of Science, Universidad Nacional de Educacion a Distancia (UNED), 28232, Las Rozas, Madrid, Spain. rplanello@ccia.uned.es. Biology and Environmental Toxicology Group, Faculty of Science, Universidad Nacional de Educacion a Distancia (UNED), 28232, Las Rozas, Madrid, Spain. Biodiversity, Ecology and Evolution Department, Complutense University of Madrid, Jose Antonio Novais, 12, 28040, Madrid, Spain. Institute for Subtropical and Mediterranean Horticulture (IHSM-UMA-CSIC), Spanish National Research Council (CSIC), Finca Experimental La Mayora, Algarrobo-Costa, 29750, Malaga, Spain. Area of Botany, Faculty of Biological and Environmental Sciences, University of Leon, 24071, Leon, Spain. Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium" |
| DOI: | 10.1038/s41598-022-24490-1 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Understanding the responses of insect herbivores to plant chemical defences is pivotal for the management of crops and pests. However, the mechanisms of interaction are not entirely understood. In this study, we compared the whole transcriptome gene expression of the aphid Macrosiphum euphorbiae grown on two different varieties of tomato that differ in their inducible chemical defences. We used two isogenic lines of tomato with a shared genetic background that only differ in the presence of type IV glandular trichomes and their associated acylsucrose excretions. This works also reports a de novo transcriptome of the aphid M. euphorbiae. Subsequently, we identified a unique and distinct gene expression profile for the first time corresponding to aphid s exposure to type IV glandular trichomes and acylsugars. The analysis of the aphid transcriptome shows that tomato glandular trichomes and their associated secretions are highly efficient in triggering stress-related responses in the aphid, and demonstrating that their role in plant defence goes beyond the physical impediment of herbivore activity. Some of the differentially expressed genes were associated with carbohydrate, lipid and xenobiotic metabolisms, immune system, oxidative stress response and hormone biosynthesis pathways. Also, the observed responses are compatible with a starvation syndrome. The transcriptome analysis puts forward a wide range of genes involved in the synthesis and regulation of detoxification enzymes that reveal important underlying mechanisms in the interaction of the aphid with its host plant and provides a valuable genomic resource for future study of biological processes at the molecular level using this aphid" |
| Keywords: | Animals *Aphids/genetics *Solanum lycopersicum/genetics/metabolism Trichomes/genetics Gene Expression Profiling Herbivory; |
| Notes: | "MedlinePlanello, Rosario Llorente, Lola Herrero, Oscar Novo, Marta Blanco-Sanchez, Lidia Diaz-Pendon, Juan Antonio Fernandez-Munoz, Rafael Ferrero, Victoria de la Pena, Eduardo eng AGL2015-67733-R./Ministerio de Economia y Competitividad, Spain/ ID 100010434, CODE: LCF/BQ/DE16/11570012/'la Caixa' Foundation/ Research Support, Non-U.S. Gov't England 2022/11/24 Sci Rep. 2022 Nov 23; 12(1):20154. doi: 10.1038/s41598-022-24490-1" |
