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Insect Biochem Mol Biol

Title:		Sequencing and characterizing odorant receptors of the cerambycid beetle Megacyllene caryae
Author(s):		Mitchell RF; Hughes DT; Luetje CW; Millar JG; Soriano-Agaton F; Hanks LM; Robertson HM;
Address:		"Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA"
Journal Title:		Insect Biochem Mol Biol
Year:		2012
Volume:		20120405
Issue:		7
Page Number:		499 - 505
DOI:		10.1016/j.ibmb.2012.03.007
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"Odorant receptors (Ors) are a unique family of ligand-gated ion channels and the primary mechanism by which insects detect volatile chemicals. Here, we describe 57 putative Ors sequenced from an antennal transcriptome of the cerambycid beetle Megacyllene caryae (Gahan). The male beetles produce a pheromone blend of nine compovnents, and we functionally characterized Ors tuned to three of these chemicals: receptor McOr3 is sensitive to (S)-2-methyl-1-butanol; McOr20 is sensitive to (2S,3R)-2,3-hexanediol; and McOr5 is sensitive to 2-phenylethanol. McOr3 and McOr20 are also sensitive to structurally-related chemicals that are pheromones of other cerambycid beetles, suggesting that orthologous receptors may be present across many cerambycid species. These Ors are the first to be functionally characterized from any species of beetle and lay the groundwork for understanding the evolution of pheromones within the Cerambycidae"
Keywords:		Animals Arthropod Antennae/drug effects/metabolism Coleoptera/*genetics/metabolism Female Gene Expression Regulation Glycols/pharmacology Insect Proteins/*genetics/metabolism Ligand-Gated Ion Channels/metabolism Male Molecular Sequence Annotation Oocytes;
Notes:		"MedlineMitchell, Robert F Hughes, David T Luetje, Charles W Millar, Jocelyn G Soriano-Agaton, Flor Hanks, Lawrence M Robertson, Hugh M eng R01 DC011091/DC/NIDCD NIH HHS/ R01 DC011091-01/DC/NIDCD NIH HHS/ T32 NS007044/NS/NINDS NIH HHS/ T32 NS007044-23/NS/NINDS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2012/04/17 Insect Biochem Mol Biol. 2012 Jul; 42(7):499-505. doi: 10.1016/j.ibmb.2012.03.007. Epub 2012 Apr 5"