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J Clin Microbiol

Title:		"Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies"
Author(s):		Chaturvedi S; Rodeghier B; Fan J; McClelland CM; Wickes BL; Chaturvedi V;
Address:		"Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA"
Journal Title:		J Clin Microbiol
Year:		2000
Volume:		38
Issue:		5
Page Number:		2007 - 2009
DOI:		10.1128/JCM.38.5.2007-2009.2000
ISSN/ISBN:		0095-1137 (Print) 1098-660X (Electronic) 0095-1137 (Linking)
Abstract:		"Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry"
Keywords:		"Cryptococcosis/*epidemiology Cryptococcus neoformans/*classification/*genetics Humans Molecular Epidemiology Pheromones/*genetics Ploidies Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Restriction Mapping;"
Notes:		"MedlineChaturvedi, S Rodeghier, B Fan, J McClelland, C M Wickes, B L Chaturvedi, V eng R29 AI043522/AI/NIAID NIH HHS/ R29-AI-41968/AI/NIAID NIH HHS/ R29-AI-43522/AI/NIAID NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2000/05/02 J Clin Microbiol. 2000 May; 38(5):2007-9. doi: 10.1128/JCM.38.5.2007-2009.2000"