| Title: | Plant genetic relatedness and volatile-mediated signalling between Solanum tuberosum plants in response to herbivory by Spodoptera exigua |
| Author(s): | Martin-Cacheda L; Vazquez-Gonzalez C; Rasmann S; Roder G; Abdala-Roberts L; Moreira X; |
| Address: | "Mision Biologica de Galicia (MBG-CSIC), Apartado de correos 28, 36080 Pontevedra, Galicia, Spain. Electronic address: luciamartincacheda.93@gmail.com. Mision Biologica de Galicia (MBG-CSIC), Apartado de correos 28, 36080 Pontevedra, Galicia, Spain; Department of Ecology and Evolutionary Biology, University of California-Irvine, 92697 Irvine, California, USA. Institute of Biology, University of Neuchatel, Rue Emile-Argand 11, 2000 Neuchatel, Switzerland. Departamento de Ecologia Tropical, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan, Apartado Postal 4-116, Itzimna. 97000. Merida, Yucatan, Mexico. Mision Biologica de Galicia (MBG-CSIC), Apartado de correos 28, 36080 Pontevedra, Galicia, Spain. Electronic address: xmoreira1@gmail.com" |
| DOI: | 10.1016/j.phytochem.2022.113561 |
| ISSN/ISBN: | 1873-3700 (Electronic) 0031-9422 (Linking) |
| Abstract: | "It has been proposed that plant-plant signalling via herbivore-induced volatile organic compounds (VOCs) should be stronger between closely related than unrelated plants. However, empirical tests remain limited and few studies have provided detailed assessments of induced changes in VOCs emissions across plant genotypes to explain genetic relatedness effects. In this study, we tested whether airborne signalling in response to herbivory between Solanum tuberosum (potato) plants was contingent on plant genetic relatedness, and further investigated genotypic variation in VOCs potentially underlying signalling and its contingency on relatedness. We carried out a greenhouse experiment using 15 S. tuberosum varieties placing pairs of plants in plastic cages, i.e. an emitter and a receiver, where both plants were of the same genotype or different genotype thereby testing for self-recognition, an elemental form genetic relatedness effects. Then, for half of the cages within each level of relatedness the emitter plant was damaged by Spodoptera exigua larvae whereas for the other half the emitter was not damaged. Three days later, we placed S. exigua larvae on receivers to test for emitter VOC effects on leaf consumption and larval weight gain (i.e. induced resistance). In addition, we used a second group of plants subjected to the same induction treatment with the same S. tuberosum varieties to test for herbivore-induced changes in VOC emissions and variation in VOC emissions among these plant genotypes. We found that herbivory drove changes in VOC composition but not total emissions, and also observed quantitative and qualitative variation in constitutive and induced VOC emissions among varieties. Results from the bioassay showed that the amount of leaf area consumed and larval weight gain on receiver plants exposed to damaged emitters were significantly lower compared to mean values on receivers exposed to control emitters. However, and despite genotypic variation in induced VOCs, this signalling effect was not contingent on plant genetic relatedness. These findings provide evidence of VOCs-mediated signalling between S. tuberosum plants in response to S. exigua damage, but no evidence of self-recognition effects in signalling contingent on variation in VOC emissions among S. tuberosum varieties" |
| Keywords: | Animals Herbivory Spodoptera *Solanum tuberosum/genetics *Volatile Organic Compounds Plant Leaves Noctuidae Plant-herbivore interactions Plant-plant communication Solanaceae Spodoptera exigua Solanum tuberosum Volatile organic compounds; |
| Notes: | "MedlineMartin-Cacheda, Lucia Vazquez-Gonzalez, Carla Rasmann, Sergio Roder, Gregory Abdala-Roberts, Luis Moreira, Xoaquin eng England 2022/12/14 Phytochemistry. 2023 Feb; 206:113561. doi: 10.1016/j.phytochem.2022.113561. Epub 2022 Dec 10" |
