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PLoS One

Title:		"Overexpression of the aphid-induced serine protease inhibitor CI2c gene in barley affects the generalist green peach aphid, not the specialist bird cherry-oat aphid"
Author(s):		Losvik A; Beste L; Stephens J; Jonsson L;
Address:		"Department of Ecology, Environment and Plant Sciences, Stockholm University, Stockholm, Sweden. Cell and Molecular Science, James Hutton Institute, Invergowrie, Dundee, United Kingdom"
Journal Title:		PLoS One
Year:		2018
Volume:		20180319
Issue:		3
Page Number:		e0193816 -
DOI:		10.1371/journal.pone.0193816
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Aphids are serious pests in crop plants. In an effort to identify plant genes controlling resistance against aphids, we have here studied a protease inhibitor, CI2c in barley (Hordeum vulgare L.). The CI2c gene was earlier shown to be upregulated by herbivory of the bird cherry-oat aphid (Rhopalosiphum padi L.) in barley genotypes with moderate resistance against this aphid, but not in susceptible lines. We hypothesized that CI2c contributes to the resistance. To test this idea, cDNA encoding CI2c was overexpressed in barley and bioassays were carried out with R. padi. For comparison, tests were carried out with the green peach aphid (Myzus persicae Sulzer), for which barley is a poor host. The performance of R. padi was not different on the CI2c-overexpressing lines in comparison to controls in test monitoring behavior and fecundity. M. persicae preference was affected as shown in the choice test, this species moved away from control plants, but remained on the CI2c-overexpressing lines. R. padi-induced responses related to defense were repressed in the overexpressing lines as compared to in control plants or the moderately resistant genotypes. A putative susceptibility gene, coding for a beta-1,3-glucanase was more strongly induced by aphids in one of the CI2c-overexpressing lines. The results indicate that the CI2c inhibitor in overexpressing lines affects aphid-induced responses by suppressing defense. This is of little consequence to the specialist R.padi, but causes lower non-host resistance towards the generalist M. persicae in barley"
Keywords:		"Animals Aphids/*physiology Avoidance Learning Chymotrypsin/metabolism Fertility Herbivory/physiology Hordeum/genetics/*metabolism Host-Parasite Interactions Peptide Hydrolases/metabolism Plant Leaves/metabolism Plant Proteins/genetics/*metabolism Plants, ;"
Notes:		"MedlineLosvik, Aleksandra Beste, Lisa Stephens, Jennifer Jonsson, Lisbeth eng Research Support, Non-U.S. Gov't 2018/03/20 PLoS One. 2018 Mar 19; 13(3):e0193816. doi: 10.1371/journal.pone.0193816. eCollection 2018"