| Title: | Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry |
| Author(s): | Li Z; Li Y; Zhan L; Meng L; Huang X; Wang T; Li Y; Nie Z; |
| Address: | "Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. University of Chinese Academy of Sciences, Beijing 100049, China. Institute of Cell Analysis, Shenzhen Bay Laboratory, Shenzhen 518132, China" |
| DOI: | 10.1021/acs.analchem.1c01011 |
| ISSN/ISBN: | 1520-6882 (Electronic) 0003-2700 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. A probe molecule, 4-aminothiophenol (4-ATP), was anchored on a paper substrate to specifically capture gas-phase aldehydes through the Schiff base reaction. Meanwhile, thin-film reaction acceleration was utilized to increase capture efficiency. By directly coupling the test paper to a mass spectrometer through paper spray, high sensitivity (0.1 ppt) and a wide quantification linear range (from 10 ppt to 1 ppm) were obtained. Analysis of EB from lung cancer patients with the test paper showed a significant increase in several reported aldehyde markers compared to EB from healthy volunteers, indicating the potential of this method for sensitive, low-cost, and convenient lung cancer screening and diagnosis" |
| Keywords: | Aldehydes Breath Tests Early Detection of Cancer Exhalation Humans *Lung Neoplasms/diagnosis Mass Spectrometry Point-of-Care Testing *Volatile Organic Compounds; |
| Notes: | "MedlineLi, Zhengzhou Li, Yuze Zhan, Lingpeng Meng, Lingwei Huang, Xi Wang, Tie Li, Yafeng Nie, Zongxiu eng Research Support, Non-U.S. Gov't 2021/06/25 Anal Chem. 2021 Jul 6; 93(26):9158-9165. doi: 10.1021/acs.analchem.1c01011. Epub 2021 Jun 24" |
