| Title: | Urinary metabolic profiling of volatile organic compounds in acute exposed volunteers after an oil spill in Republic of Korea |
| Author(s): | Lee J; Kim MH; Ha M; Chung BC; |
| Address: | "Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "The Herbei Spirit oil spill occurred in western Korea. A large number of people who participated in the cleanup tasks of the contaminated area were exposed to crude oil component. We developed a method to monitor volatile organic compound (VOC) metabolites in urine, and evaluate the acute exposure caused by the oil spill in exposed volunteer workers (n = 100, 20.7 +/- 2.1 years, mean +/- SD). Acidified urine samples were extracted by SPE, derivatized with trimethylsilyl, and analyzed using gas chromatography-mass spectrometry. Calibration curves were found to be linear from 3 to 1000 ng/mL (r(2) > 0.993). Accuracy was over 82.4%, and precision was less than 24.8%. Using this method, the VOC metabolites, except hippuric acid, were present at higher levels in the urine samples of volunteers after cleanup work. The levels of mandelic acid (MA) and trans,trans-muconic acid (t,t-MU) were increased significantly (p < 0.001). The exposure effect was greater in women than in men. The effect of smoking was analyzed in all exposed and non-exposed groups, with non-smokers showing increased MA and t,t-MU levels related to exposure. The present method was reliable to determine VOC metabolites in urine and could be useful for biomonitoring of acute exposure effects of VOCs" |
| Keywords: | Adolescent Adult Calibration Female Gas Chromatography-Mass Spectrometry/*methods Humans Male Occupational Exposure/*analysis Petroleum/*analysis/*metabolism Republic of Korea Sensitivity and Specificity Volatile Organic Compounds/*metabolism/*urine Young; |
| Notes: | "MedlineLee, Jeongae Kim, Min-hwa Ha, Mina Chung, Bong Chul eng Research Support, Non-U.S. Gov't Validation Study England 2009/10/02 Biomed Chromatogr. 2010 May; 24(5):562-8. doi: 10.1002/bmc.1328" |
