| Title: | Rapid modulation of gene expression profiles in the telencephalon of male goldfish following exposure to waterborne sex pheromones |
| Author(s): | Lado WE; Zhang D; Mennigen JA; Zamora JM; Popesku JT; Trudeau VL; |
| Address: | "Department of Biology, University of Ottawa, Ottawa, Canada" |
| DOI: | 10.1016/j.ygcen.2013.06.015 |
| ISSN/ISBN: | 1095-6840 (Electronic) 0016-6480 (Linking) |
| Abstract: | "Sex pheromones rapidly affect endocrine physiology and behaviour, but little is known about their effects on gene expression in the neural tissues that mediate olfactory processing. In this study, we exposed male goldfish for 6h to waterborne 17,20betaP (4.3 nM) and PGF2alpha (3 nM), the main pre-ovulatory and post-ovulatory pheromones, respectively. Both treatments elevated milt volume (P=0.001). Microarray analysis of male telencephalon following PGF2alpha treatment identified 71 unique transcripts that were differentially expressed (q<5%; 67 up, 4 down). Functional annotation of these regulated genes indicates that PGF2alpha pheromone exposure affects diverse biological processes including nervous system functions, energy metabolism, cholesterol/lipoprotein transport, translational regulation, transcription and chromatin remodelling, protein processing, cytoskeletal organization, and signalling. By using real-time RT-PCR, we further validated three candidate genes, ependymin-II, calmodulin-A and aldolase C, which exhibited 3-5-fold increase in expression following PGF2alpha exposure. Expression levels of some other genes that are thought to be important for reproduction were also determined using real-time RT-PCR. Expression of sGnRH was increased by PGF2alpha, but not 17,20betaP, whereas cGnRH expression was increased by 17,20betaP but not PGF2alpha. In contrast, both pheromones increase the expression of glutamate (GluR2a, NR2A) and gamma-aminobutyric acid (GABAA gamma2) receptor subunit mRNAs. Milt release and rapid modulation of neuronal transcription are part of the response of males to female sex pheromones" |
| Keywords: | Animals Dinoprost/pharmacology Female Goldfish/*metabolism Male Sex Attractants/*pharmacology Telencephalon/*drug effects/*metabolism Aldolase C Calmodulin Ependymin GnRH Microarray Pheromones Sperm release; |
| Notes: | "MedlineLado, Wudu E Zhang, Dapeng Mennigen, Jan A Zamora, Jacob M Popesku, Jason T Trudeau, Vance L eng Research Support, Non-U.S. Gov't 2013/06/27 Gen Comp Endocrinol. 2013 Oct 1; 192:204-13. doi: 10.1016/j.ygcen.2013.06.015. Epub 2013 Jun 22" |
