| Title: | Volatiles formation from grape must fermentation using a cryophilic and thermotolerant yeast |
| Author(s): | Kopsahelis N; Bosnea L; Kanellaki M; Koutinas AA; |
| Address: | "Food Biotechnology Group, Section of Analytical Environmental and Applied Chemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece. Kopsahelis@upatras.gr" |
| DOI: | 10.1007/s12010-012-9582-2 |
| ISSN/ISBN: | 1559-0291 (Electronic) 0273-2289 (Linking) |
| Abstract: | "Grape must fermentation performance and volatiles formation by simultaneously cryophilic and thermotolerant yeast (strain AXAZ-1), isolated from grapes in Greece, was evaluated in a wide temperature range (5-40 degrees C). Yeast strain was immobilized on brewer's spent grains (BSG) and the formed biocatalyst was introduced into a Multi-Stage Fixed Bed Tower (MFBT) bioreactor. Almost complete sugar utilization from the aforementioned biocatalyst was observed in a wide temperature spectrum, ranging from 5 degrees C to 37 degrees C, while at 40 degrees C residual sugar was up to 29 g/l. Time to complete fermentation with the immobilized yeast ranged from 290 h at 5 degrees C and 120 h at 40 degrees C to 25 h at 33 degrees C. The daily ethanol productivity reached maximum (88.6 g/l) and minimum (5.6 g/l) levels at 33 degrees C and 5 degrees C, respectively. The aroma-related compounds' profiles of immobilized cells at different fermentation temperatures were evaluated by using solid phase microextraction (SPME) gas chromatography/mass spectrometry (GC/MS). Must fermentation resulted in a high-quality fermentation product due to the low concentrations of higher and amyl alcohols at all temperatures tested. AXAZ-1 is a very promising strain for quality wine production, as it is capable of performing fermentations of high ethanol concentration and productivities in both low and high temperatures" |
| Keywords: | "Beverages/*microbiology Bioreactors/microbiology Cells, Immobilized/metabolism *Cold Temperature *Fermentation *Hot Temperature Saccharomyces cerevisiae/cytology/*metabolism/physiology *Vitis Volatile Organic Compounds/*metabolism;" |
| Notes: | "MedlineKopsahelis, Nikolaos Bosnea, Loulouda Kanellaki, Maria Koutinas, Athanasios A eng Research Support, Non-U.S. Gov't 2012/02/14 Appl Biochem Biotechnol. 2012 Jul; 167(5):1183-98. doi: 10.1007/s12010-012-9582-2. Epub 2012 Feb 11" |
