| Title: | "Sex pheromone production in the silkworm, Bombyx mori, is mediated by store-operated Ca2+ channels" |
| Author(s): | Hull JJ; Kajigaya R; Imai K; Matsumoto S; |
| Address: | "Molecular Entomology Laboratory, RIKEN, Wako, Saitama, Japan. joeh@postman.riken.go.jp" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 0916-8451 (Print) 0916-8451 (Linking) |
| Abstract: | "In most female moths, pheromone biosynthesis activating neuropeptide (PBAN) regulates sex pheromone production by stimulating an influx of extracellular Ca(2+). Little is known about the plasma membrane channel or how the PBAN stimulus is communicated to the channel. Fluorescent Ca(2+) imaging techniques confirmed PBAN-induced Ca(2+) influx in the silkworm, Bombyx mori, and showed that the PBAN response is reduced with repeated stimulation. Compounds known to impact Ca(2+) signaling were examined for their effects on sex pheromone production. These experiments demonstrated that the PBAN signal is likely mediated by a store-operated channel (SOC). SOC blockers, SKF-96365 and 2-aminoethoxydiphenyl borate, abolished sex pheromone production, as did flufenamic acid, a blocker of transient receptor potential (TRP) channels. Thapsigargin mimicked the pheromonotropic effects of PBAN. Similar results were seen when PBAN-induced lipase activity was assayed. Conversely, 1-oleoyl-2-acetyl-sn-glycerol and arachidonic acid, activators of diacylglycerol-dependent Ca(2+) channels, had no effect on bombykol production" |
| Keywords: | Animals Bombyx Calcium Channel Agonists/pharmacology Calcium Channel Blockers/pharmacology Calcium Channels/*physiology Calcium Signaling/physiology Female Neuropeptides/*physiology Sex Attractants/*biosynthesis; |
| Notes: | "MedlineHull, J Joe Kajigaya, Ryosuke Imai, Kiyohiro Matsumoto, Shogo eng Research Support, Non-U.S. Gov't England 2007/08/11 Biosci Biotechnol Biochem. 2007 Aug; 71(8):1993-2001. doi: 10.1271/bbb.70175. Epub 2007 Aug 7" |
