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Methods Mol Biol

Title:		Synchronization of the Budding Yeast Saccharomyces cerevisiae
Author(s):		Foltman M; Molist I; Sanchez-Diaz A;
Address:		"Instituto de Biomedicina y Biotecnologia de Cantabria, Consejo Superior de Investigaciones Cientificas/Universidad de Cantabria, Albert Einstein 22, 39011, Santander, Spain. Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, 39011, Santander, Spain. Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, 39011, Santander, Spain. a.sanchezdiaz@unican.es. Instituto de Biomedicina y Biotecnologia de Cantabria, Consejo Superior de Investigaciones Cientificas, Universidad de Cantabria, Albert Einstein 22, 39011, Santander, Spain. a.sanchezdiaz@unican.es"
Journal Title:		Methods Mol Biol
Year:		2016
Volume:		1369
Issue:
Page Number:		279 - 291
DOI:		10.1007/978-1-4939-3145-3_19
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"A number of model organisms have provided the basis for our understanding of the eukaryotic cell cycle. These model organisms are generally much easier to manipulate than mammalian cells and as such provide amenable tools for extensive genetic and biochemical analysis. One of the most common model organisms used to study the cell cycle is the budding yeast Saccharomyces cerevisiae. This model provides the ability to synchronise cells efficiently at different stages of the cell cycle, which in turn opens up the possibility for extensive and detailed study of mechanisms regulating the eukaryotic cell cycle. Here, we describe methods in which budding yeast cells are arrested at a particular phase of the cell cycle and then released from the block, permitting the study of molecular mechanisms that drive the progression through the cell cycle"
Keywords:		*Cell Cycle/drug effects Cell Cycle Proteins/metabolism Flow Cytometry GTP-Binding Proteins/metabolism Hydroxyurea/pharmacology Mating Factor Nocodazole/pharmacology Peptides/metabolism Saccharomyces cerevisiae/drug effects/*physiology Alpha factor Buddin;
Notes:		"MedlineFoltman, Magdalena Molist, Iago Sanchez-Diaz, Alberto eng Research Support, Non-U.S. Gov't 2015/11/01 Methods Mol Biol. 2016; 1369:279-91. doi: 10.1007/978-1-4939-3145-3_19"