| Title: | Purification to apparent homogeneity of the mating pheromone of mat-1 homozygous Euplotes raikovi |
| Author(s): | Concetti A; Raffioni S; Miceli C; Barra D; Luporini P; |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "Mating type-specific mating pheromones autonomously released into the environment control cell-cell union in conjugation of the marine ciliate Euplotes raikovi. The mating pheromone, termed euplomone r-1, of cells of the homozygous mating type determined by the mat-1/mat-1 genotype was purified by means of a three-stage purification procedure which provides a yield of 79%. Starting from 10 liters of supernatant, 3.3 mg of euplomone r-1 were regularly recovered. Euplomone r-1 was identified with a protein showing a molecular weight of 12,000, an isoelectric point of 3.7, and unusually high contents of cysteine (15%) and tyrosine (5.8%). It appeared active at a concentration of 3.4 +/- 0.5 X 10(-12) M. Carbohydrate and a small colored compound, not yet identified, occurred in association with partially purified euplomone r-1 samples, whereas they were not detected in samples of the final product of purification. An acidic shift was apparently capable of destroying the carbohydrate/euplomone r-1 association, suggesting that it does not involve covalent bonds" |
| Keywords: | Amino Acids/analysis Animals Carbohydrates/analysis Ciliophora/*physiology Homozygote Isoelectric Focusing Mating Factor Molecular Weight Peptides/*isolation & purification Pheromones/*isolation & purification; |
| Notes: | "MedlineConcetti, A Raffioni, S Miceli, C Barra, D Luporini, P eng Research Support, Non-U.S. Gov't 1986/08/15 J Biol Chem. 1986 Aug 15; 261(23):10582-6" |
