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Microbiol Res

Title:		Proteomic response of the phytopathogen Phyllosticta citricarpa to antimicrobial volatile organic compounds from Saccharomyces cerevisiae
Author(s):		Fialho MB; de Andrade A; Bonatto JM; Salvato F; Labate CA; Pascholati SF;
Address:		"Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de Sao Paulo (USP), Avenida Padua Dias, 11, CP 09, CEP 13418-900 Piracicaba, SP, Brazil. Departamento de Genetica, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de Sao Paulo (USP), Avenida Padua Dias, 11, CP 09, CEP 13418-900 Piracicaba, SP, Brazil. Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de Sao Paulo (USP), Avenida Padua Dias, 11, CP 09, CEP 13418-900 Piracicaba, SP, Brazil. Electronic address: sfpascho@usp.br"
Journal Title:		Microbiol Res
Year:		2016
Volume:		20151201
Issue:
Page Number:		1 - 7
DOI:		10.1016/j.micres.2015.11.002
ISSN/ISBN:		1618-0623 (Electronic) 0944-5013 (Linking)
Abstract:		"Volatile organic compounds (VOCs) released by Saccharomyces cerevisiae inhibit plant pathogens, including the filamentous fungus Phyllosticta citricarpa, causal agent of citrus black spot. VOCs mediate relevant interactions between organisms in nature, and antimicrobial VOCs are promising, environmentally safer fumigants to control phytopathogens. As the mechanisms by which VOCs inhibit microorganisms are not well characterized, we evaluated the proteomic response in P. citricarpa after exposure for 12h to a reconstituted mixture of VOCs (alcohols and esters) originally identified in S. cerevisiae. Total protein was extracted and separated by 2D-PAGE, and differentially expressed proteins were identified by LC-MS/MS. About 600 proteins were detected, of which 29 were downregulated and 11 were upregulated. These proteins are involved in metabolism, genetic information processing, cellular processes, and transport. Enzymes related to energy-generating pathways, particularly glycolysis and the tricarboxylic acid cycle, were the most strongly affected. Thus, the data indicate that antimicrobial VOCs interfere with essential metabolic pathways in P. citricarpa to prevent fungal growth"
Keywords:		"Amino Acids/metabolism Anti-Infective Agents/*pharmacology Antifungal Agents/chemistry/metabolism/pharmacology Ascomycota/*drug effects/genetics/metabolism Citric Acid Cycle/drug effects Citrus/microbiology Electrophoresis, Gel, Two-Dimensional Fungal Pro;"
Notes:		"MedlineFialho, Mauricio Batista de Andrade, Alexander Bonatto, Jose Matheus Camargo Salvato, Fernanda Labate, Carlos Alberto Pascholati, Sergio Florentino eng Research Support, Non-U.S. Gov't Germany 2016/01/26 Microbiol Res. 2016 Feb; 183:1-7. doi: 10.1016/j.micres.2015.11.002. Epub 2015 Dec 1"