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Cell Rep

Title:		Stimulation of medial amygdala GABA neurons with kinetically different channelrhodopsins yields opposite behavioral outcomes
Author(s):		Baleisyte A; Schneggenburger R; Kochubey O;
Address:		"Laboratory of Synaptic Mechanisms, Brain Mind Institute, School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Laboratory of Synaptic Mechanisms, Brain Mind Institute, School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address: ralf.schneggenburger@epfl.ch. Laboratory of Synaptic Mechanisms, Brain Mind Institute, School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address: olexiy.kochubey@epfl.ch"
Journal Title:		Cell Rep
Year:		2022
Volume:		39
Issue:		8
Page Number:		110850 -
DOI:		10.1016/j.celrep.2022.110850
ISSN/ISBN:		2211-1247 (Electronic)
Abstract:		"The medial amygdala (MeA) receives pheromone information about conspecifics and has crucial functions in social behaviors. A previous study showed that activation of GABA neurons in the postero-dorsal MeA (MeApd) with channelrhodopsin-2(H134R) (ChR2) stimulates inter-male aggression. When performing these experiments using the faster channelrhodopsin(H134R,E123T) (ChETA), we find the opposite behavioral outcome. A systematic comparison between the two channelrhodopsin variants reveals that optogenetic activation of MeApd GABA neurons with ChETA suppresses aggression, whereas activation under ChR2 increases aggression. Although the mechanism for this paradoxical difference is not understood, we observe that activation of MeApd GABA neurons with ChR2 causes larger plateau depolarizations, smaller action potentials, and larger local inhibition than with ChETA. Thus, the channelrhodopsin variant used for in vivo optogenetic experiments can radically influence the behavioral outcome. Future work should continue to study the role of specific sub-populations of MeApd GABA neurons in aggression control"
Keywords:		Action Potentials/physiology Amygdala Channelrhodopsins/genetics *GABAergic Neurons Humans Male *Optogenetics CP: Neuroscience aggression channelrhodopsin inhibitory neurons medial amygdala optogenetics resident-intruder test social behavior;
Notes:		"MedlineBaleisyte, Aiste Schneggenburger, Ralf Kochubey, Olexiy eng Research Support, Non-U.S. Gov't 2022/05/26 Cell Rep. 2022 May 24; 39(8):110850. doi: 10.1016/j.celrep.2022.110850"