« Previous Abstract[Allelopathic effects of grafted eggplant root exudates]    Next Abstract"Bacterial symbionts, Buchnera, and starvation on wing dimorphism in English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae)" »

FEMS Microbiol Lett

Title:		Fusarium oxysporum induces the production of proteins and volatile organic compounds by Trichoderma harzianum T-E5
Author(s):		Zhang F; Yang X; Ran W; Shen Q;
Address:		"National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing, China"
Journal Title:		FEMS Microbiol Lett
Year:		2014
Volume:		20140908
Issue:		1
Page Number:		116 - 123
DOI:		10.1111/1574-6968.12582
ISSN/ISBN:		1574-6968 (Electronic) 0378-1097 (Linking)
Abstract:		"Trichoderma species have been used widely as biocontrol agents for the suppression of soil-borne pathogens. However, some antagonistic mechanisms of Trichoderma are not well characterized. In this study, a series of laboratory experiments were designed to characterize the importance of mycoparasitism, exoenzymes, and volatile organic compounds (VOCs) by Trichoderma harzianum T-E5 for the control of Fusarium oxysporum f. sp. cucumerinum (FOC). We further tested whether these mechanisms were inducible and upregulated in presence of FOC. The results were as follows: T-E5 heavily parasitized FOC by coiling and twisting the entire mycelium of the pathogen in dual cultures. T-E5 growing medium conditioned with deactivated FOC (T2) showed more proteins and higher cell wall-degrading enzyme activities than T1, suggesting that FOC could induce the upregulation of exoenzymes. The presence of deactivated FOC (T2') also resulted in the upregulation of VOCs that five and eight different types T-E5-derived VOCs were identified from T1' and T2', respectively. Further, the excreted VOCs in T2' showed significantly higher antifungal activities against FOC than T1'. In conclusion, mycoparasitism of T-E5 against FOC involved mycelium contact and the production of complex extracellular substances. Together, these data provide clues to help further clarify the interactions between these fungi"
Keywords:		Antifungal Agents/metabolism Fungal Proteins/*metabolism Fusarium/*growth & development *Microbial Interactions Trichoderma/*growth & development/*metabolism Volatile Organic Compounds/*metabolism headspace solid-phase micro-extraction mycoparasitism prot;
Notes:		"MedlineZhang, Fengge Yang, Xingming Ran, Wei Shen, Qirong eng Research Support, Non-U.S. Gov't England 2014/08/20 FEMS Microbiol Lett. 2014 Oct; 359(1):116-23. doi: 10.1111/1574-6968.12582. Epub 2014 Sep 8"