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PLoS One

Title:		Odorant-dependent generation of nitric oxide in Mammalian olfactory sensory neurons
Author(s):		Brunert D; Kurtenbach S; Isik S; Benecke H; Gisselmann G; Schuhmann W; Hatt H; Wetzel CH;
Address:		"Lehrstuhl fur Zellphysiologie, Ruhr-Universitat Bochum, Bochum, Germany"
Journal Title:		PLoS One
Year:		2009
Volume:		20090511
Issue:		5
Page Number:		e5499 -
DOI:		10.1371/journal.pone.0005499
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"The gaseous signalling molecule nitric oxide (NO) is involved in various physiological processes including regulation of blood pressure, immunocytotoxicity and neurotransmission. In the mammalian olfactory bulb (OB), NO plays a role in the formation of olfactory memory evoked by pheromones as well as conventional odorants. While NO generated by the neuronal isoform of NO synthase (nNOS) regulates neurogenesis in the olfactory epithelium, NO has not been implicated in olfactory signal transduction. We now show the expression and function of the endothelial isoform of NO synthase (eNOS) in mature olfactory sensory neurons (OSNs) of adult mice. Using NO-sensitive micro electrodes, we show that stimulation liberates NO from isolated wild-type OSNs, but not from OSNs of eNOS deficient mice. Integrated electrophysiological recordings (electro-olfactograms or EOGs) from the olfactory epithelium of these mice show that NO plays a significant role in modulating adaptation. Evidence for the presence of eNOS in mature mammalian OSNs and its involvement in odorant adaptation implicates NO as an important new element involved in olfactory signal transduction. As a diffusible messenger, NO could also have additional functions related to cross adaptation, regeneration, and maintenance of MOE homeostasis"
Keywords:		Acyclic Monoterpenes Aldehydes/pharmacology Animals Calcium/metabolism Colforsin/pharmacology Electrophysiology Enzyme Inhibitors/pharmacology Epithelium/drug effects/metabolism/physiology Green Fluorescent Proteins/genetics/metabolism Immunohistochemistr;
Notes:		"MedlineBrunert, Daniela Kurtenbach, Stefan Isik, Sonnur Benecke, Heike Gisselmann, Gunter Schuhmann, Wolfgang Hatt, Hanns Wetzel, Christian H eng Research Support, Non-U.S. Gov't 2009/05/12 PLoS One. 2009; 4(5):e5499. doi: 10.1371/journal.pone.0005499. Epub 2009 May 11"