« Previous AbstractAllelochemicals targeted to balance competing selections in African agroecosystems    Next AbstractContinuous waste activated sludge and food waste co-fermentation for synchronously recovering vivianite and volatile fatty acids at different sludge retention times: Performance and microbial response »

Microbiologyopen

Title:		Antifungal and plant growth promotion activity of volatile organic compounds produced by Bacillus amyloliquefaciens
Author(s):		Wu Y; Zhou J; Li C; Ma Y;
Address:		"Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, China. School of Animal, Rural and Environmental Sciences, Nottingham Trent University, Nottingham, England"
Journal Title:		Microbiologyopen
Year:		2019
Volume:		20190324
Issue:		8
Page Number:		e00813 -
DOI:		10.1002/mbo3.813
ISSN/ISBN:		2045-8827 (Electronic) 2045-8827 (Linking)
Abstract:		"Fusarium wilt of watermelon, caused by F. oxysporum f.sp. niveum (FON), is a devastating disease that causes extensive losses throughout the world. Five bacterial strains (L3, h, beta, b, and L) isolated from the watermelon rhizosphere showed antagonistic activity against FON during in vitro tests. Strain L3 produced diffusible and volatile organic compounds (VOCs) which showed the strongest antifungal activity. Arabidopsis thaliana plantlets exposed to VOCs produced by strain L3 showed a 2.39-fold increase in biomass, 1.40-fold increase in primary root length, and 5.05-fold increase in number of lateral roots. Confocal laser scanning microscope showed that the GFP-labeled strain L3 could colonize along the elongation and differentiation zones of watermelon roots. In greenhouse pot experiments, the biocontrol efficiency of strain L3 against fusarium wilt of watermelon was up to 68.4% in comparison with the control treatment. In addition, inoculation of the strain L3 resulted in a 23.4% increase in plant fresh weight. Based on 16S rDNA sequence analysis, the strain L3 was identified as Bacillus amyloliquefaciens L3. Fourteen VOCs produced by strain L3 were identified through GC-MS analysis. Of nine VOCs tested, 2-nonanone and 2-heptanone were proved to have strong antifungal properties. Acetoin and 2,3-butanediol were found to promote plant growth. The results suggested B. amyloliquefaciens L3 was a potential biocontrol agent, and that VOCs produced by B. amyloliquefaciens L3 play important roles in the process of biocontrol and plant growth promotion"
Keywords:		"Antifungal Agents/*metabolism Arabidopsis/growth & development Bacillus amyloliquefaciens/classification/genetics/isolation & purification/*metabolism Citrullus/growth & development/microbiology Cluster Analysis DNA, Bacterial/chemistry/genetics DNA, Ribo;"
Notes:		"MedlineWu, Yuncheng Zhou, Jinyan Li, Chengguo Ma, Yan eng Research Support, Non-U.S. Gov't England 2019/03/25 Microbiologyopen. 2019 Aug; 8(8):e00813. doi: 10.1002/mbo3.813. Epub 2019 Mar 24"