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Genetics

Title:		Molecular determinants of sporulation in Ashbya gossypii
Author(s):		Wasserstrom L; Lengeler KB; Walther A; Wendland J;
Address:		"Carlsberg Laboratory, Yeast Genetics, DK-1799 Copenhagen V, Denmark"
Journal Title:		Genetics
Year:		2013
Volume:		20130705
Issue:		1
Page Number:		87 - 99
DOI:		10.1534/genetics.113.151019
ISSN/ISBN:		1943-2631 (Electronic) 0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"Regulation of development and entry into sporulation is critical for fungi to ensure survival of unfavorable environmental conditions. Here we present an analysis of gene sets regulating sporulation in the homothallic ascomycete Ashbya gossypii. Deletion of components of the conserved pheromone/starvation MAP kinase cascades, e.g., STE11 and STE7, results in increased sporulation. In kar3 mutants sporulation is severely reduced, while deletion of KAR4 as well as of homologs of central Saccharomyces cerevisiae regulators of sporulation, IME1, IME2, IME4, and NDT80, abolishes sporulation in A. gossypii. Comparison of RNAseq transcript profiles of sporulation-deficient mutants identified a set of 67 down-regulated genes, most of which were up-regulated in the oversporulating ste12 mutant. One of these differentially expressed genes is an endoglucanase encoded by ENG2. We found that Eng2p promotes hyphal fragmentation as part of the developmental program of sporulation, which generates single-celled sporangia. Sporulation-deficient strains are arrested in their development but form sporangia. Supply of new nutrients enabled sporangia to return to hyphal growth, indicating that these cells are not locked in meiosis. Double-strand break (DSB) formation by Spo11 is apparently not required for sporulation; however, the absence of DMC1, which repairs DSBs in S. cerevisiae, results in very poor sporulation in A. gossypii. We present a comprehensive analysis of the gene repertoire governing sporulation in A. gossypii and suggest an altered regulation of IME1 expression compared to S. cerevisiae"
Keywords:		"Fungal Proteins/genetics/metabolism Gene Expression Regulation, Fungal MAP Kinase Signaling System Meiosis/*genetics Saccharomycetales/*genetics/metabolism/physiology Spores, Fungal/*genetics Transcription, Genetic Ime1 MAP kinase cascade RNAseq Ste12 mei;"
Notes:		"MedlineWasserstrom, Lisa Lengeler, Klaus B Walther, Andrea Wendland, Jurgen eng Research Support, Non-U.S. Gov't 2013/07/09 Genetics. 2013 Sep; 195(1):87-99. doi: 10.1534/genetics.113.151019. Epub 2013 Jul 5"