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Proc Natl Acad Sci U S A

Title:		The cyclin-dependent kinase inhibitor p40SIC1 imposes the requirement for Cln G1 cyclin function at Start
Author(s):		Tyers M;
Address:		"Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada"
Journal Title:		Proc Natl Acad Sci U S A
Year:		1996
Volume:		93
Issue:		15
Page Number:		7772 - 7776
DOI:		10.1073/pnas.93.15.7772
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"In yeast, commitment to cell division (Start) is catalyzed by activation of the Cdc28 protein kinase in late G1 phase by the Cln1, Cln2, and Cln3 G1 cyclins. The Clns are essential, rate-limiting activators of Start because cells lacking Cln function (referred to as cln-) arrest at Start and because CLN dosage modulates the timing of Start. At or shortly after Start, the development of B-type cyclin Clb-Cdc28 kinase activity and initiation of DNA replication requires the destruction of p40SIC1, a specific inhibitor of the Clb-Cdc28 kinases. I report here that cln cells are rendered viable by deletion of SIC1. Conversely, in cln1 cln2 cells, which have low CLN activity, modest increases in SIC1 gene dosage cause inviability. Deletion of SIC1 does not cause a general bypass of Start since (cln-)sic1 cells remain sensitive to mating pheromone-induced arrest. Far1, a pheromone-activated inhibitor of Cln-Cdc28 kinases, is dispensable for arrest of (cln-)sic1 cells by pheromone, implying the existence of an alternate Far1-independent arrest pathway. These observations define a pheromone-sensitive activity able to catalyze Start only in the absence of p40SIC1. The existence of this activity means that the B-type cyclin inhibitor p40SIC1 imposes the requirement for Cln function at Start"
Keywords:		"CDC28 Protein Kinase, S cerevisiae/antagonists & inhibitors/*metabolism Cell Cycle/drug effects/*genetics Cell Division/drug effects Cyclin-Dependent Kinase Inhibitor Proteins Cyclins/*metabolism Fungal Proteins/genetics/*metabolism Galactose/metabolism G;"
Notes:		"MedlineTyers, M eng Research Support, Non-U.S. Gov't 1996/07/23 Proc Natl Acad Sci U S A. 1996 Jul 23; 93(15):7772-6. doi: 10.1073/pnas.93.15.7772"