| Title: | Expression of a kexin-like gene from the human pathogenic fungus Paracoccidioides brasiliensis in Saccharomyces cerevisiae |
| Author(s): | Torres FA; Vilaca R; Pepe De Moraes LM; Reis VC; Felipe MS; |
| Address: | "Laboratorio de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, Brazil. ftorres@unb.br" |
| DOI: | 10.1080/13693780701875827 |
| ISSN/ISBN: | 1369-3786 (Print) 1369-3786 (Linking) |
| Abstract: | "Kexin-like proteins are proteinases belonging to the subtilase family which are involved in the processing of pro-proteins to their active forms. In fungi, kexin-like proteins are involved in several important cellular processes, including mating and dimorphism. Paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis undergoes a thermo-regulated dimorphic transition which is essential for the establishment of the infection. Although the molecular mechanisms which rule this process are still unknown, several genes identified in P. brasiliensis have been implicated in dimorphism, including kex2, a kexin-like protein. In this work we have used the baker's yeast Saccharomyces cerevisiae as a host to perform heterologous expression analysis of the P. brasiliensis kex2 gene. Our data shows that kex2 can complement the functions of a S. cerevisiae kex2 mutant strain and could therefore be considered its functional homologue" |
| Keywords: | "Cloning, Molecular DNA, Fungal/analysis Gene Expression Regulation, Fungal Genes, Mating Type, Fungal Mating Factor Paracoccidioides/*genetics Peptides/metabolism Proprotein Convertases/*genetics Saccharomyces cerevisiae/*genetics Saccharomyces cerevisiae;" |
| Notes: | "MedlineTorres, Fernando Araripe Goncalves Vilaca, Rosemary Pepe De Moraes, Lidia Maria Reis, Viviane Castelo Branco Felipe, Maria Sueli Soares eng Research Support, Non-U.S. Gov't England 2008/04/17 Med Mycol. 2008 Jun; 46(4):385-8. doi: 10.1080/13693780701875827" |
