| Title: | Functional characterization of pheromone receptor candidates in codling moth Cydia pomonella (Lepidoptera: Tortricidae) |
| Author(s): | Tian K; Liu W; Feng LK; Huang TY; Wang GR; Lin KJ; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. Institute of Plant Protection, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang Uygur Autonomous Region, China. Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong Province, China" |
| ISSN/ISBN: | 1744-7917 (Electronic) 1672-9609 (Linking) |
| Abstract: | "Sex pheromones serve a critical role in Lepidopterans finding mates. Male moths perceive and react to sex pheromones emitted by conspecific females through a delicate pheromone communication system. Pheromone receptors (PRs) are the key sensory elements at the beginning of that process. The codling moth (Cydia pomnonella) is an important pome fruit pest globally and a serious invasive species in China. Pheromone-based techniques have been used successfully in monitoring and controlling this species. We conducted ribonucleic acid sequencing analysis of the codling moth antennal transcriptome and identified 66 odorant receptors (ORs) in a population from Xinjiang province, China, of which 14 were PRs, including two novel PRs (CpomOR2e and CpomOR73). Four PRs that contain full-length open reading frames (CpomOR1, OR2a, OR5, OR7) and four PRs with ligands that have not been reported previously (CpomOR1, OR2a, OR5, OR7) were selected to deorphanize in the heterologous Xenopus oocyte expression system. Specifically, we found that CpomOR2a and CpomOR5 responded to (E,E)-8, 10-dodecadien-1-yl acetate (codlemone acetate). Furthermore, CpomOR5 (EC(50) = 1.379 x 10(-8) mol/L) was much more sensitive to codlemone acetate than CpomOR2a (EC(50) = 1.663 x 10(-6) mol/L). Since codlemone acetate is an important component of C. pomonella sex pheromone, our results improve the current understanding of pheromone communication in codling moths and will be helpful for the development of pest management strategies" |
| Keywords: | "Amino Acid Sequence Animals Arthropod Antennae/*metabolism Female Insect Proteins/chemistry/*genetics/metabolism Male Moths/*genetics/metabolism Phylogeny Receptors, Pheromone/chemistry/*genetics/metabolism Sequence Alignment Cydia pomonella antennal tran;" |
| Notes: | "MedlineTian, Ke Liu, Wei Feng, Li-Kai Huang, Tian-Yu Wang, Gui-Rong Lin, Ke-Jian eng 31672033/National Natural Science Foundation of China/ 2017M611069/China postdoctoral science funding/ Australia 2020/05/06 Insect Sci. 2021 Apr; 28(2):445-456. doi: 10.1111/1744-7917.12775. Epub 2020 Jul 22" |
