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Sci Rep

Title:		Critical thresholds of 1-Octen-3-ol shape inter-species Aspergillus interactions modulating the growth and secondary metabolism
Author(s):		Singh D; Son SY; Lee CH;
Address:		"Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Korea. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Korea. chlee123@konkuk.ac.kr"
Journal Title:		Sci Rep
Year:		2020
Volume:		20200706
Issue:		1
Page Number:		11116 -
DOI:		10.1038/s41598-020-68096-x
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"In fungi, contactless interactions are mediated via the exchange of volatile organic compounds (VOCs). As these pair-wise interactions are fundamental to complex ecosystem, we examined the effects of inter-species VOCs trade-offs in Aspergillus flavus development. First, we exposed A. flavus to the A. oryzae volatilome (Treatment-1) with highest relative abundance of 1-Octen-3-ol (~ 4.53 folds) among the C-8 VOCs. Further, we examined the effects of gradient titers of 1-Octen-3-ol (Treatment-2: 100-400 ppm/day) in a range that elicits natural interactions. On 7-day, VOC-treated A. flavus displayed significantly reduced growth and sclerotial counts (p < 0.01) coupled with higher conidial density (T2(100-200 ppm/day), p < 0.01) and alpha-amylase secretion (T2(200 ppm/day), p < 0.01), compared to the untreated sets. Similar phenotypic trends except for alpha-amylases were evident for 9-day incubated A. flavus in T2. The corresponding metabolomics data displayed a clustered pattern of secondary metabolite profiles for VOC-treated A. flavus (PC1-18.03%; PC2-10.67%). Notably, a higher relative abundance of aflatoxin B1 with lower levels of most anthraquinones, indole-terpenoids, and oxylipins was evident in VOC-treated A. flavus. The observed correlations among the VOC-treatments, phenotypes, and altered metabolomes altogether suggest that the distant exposure to the gradient titers of 1-Octen-3-ol elicits an attenuated developmental response in A. flavus characterized by heightened virulence"
Keywords:		Aspergillus/classification/drug effects/*growth & development/*metabolism Metabolome/*drug effects Octanols/*pharmacology Volatile Organic Compounds/analysis/*metabolism;
Notes:		"MedlineSingh, Digar Son, Su Young Lee, Choong Hwan eng Research Support, Non-U.S. Gov't England 2020/07/08 Sci Rep. 2020 Jul 6; 10(1):11116. doi: 10.1038/s41598-020-68096-x"