« Previous AbstractEffect of gingivitis treatment on the breath of chronic periodontitis patients    Next Abstract"Guava-flavored whey beverage processed by cold plasma technology: Bioactive compounds, fatty acid profile and volatile compounds" »

Talanta

Title:		"Essential oil-based dispersive liquid-liquid microextraction for the determination of N,N-dimethyltryptamine and beta-carbolines in human plasma: A novel solvent-free alternative"
Author(s):		Silveira GO; Lourenco FR; Fonseca Pego AM; Guimaraes Dos Santos R; Rossi GN; Hallak JEC; Yonamine M;
Address:		"Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, 05508-000, Brazil. Electronic address: silveirago@usp.br. Department of Pharmacy, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, 05508-000, Brazil. Dutch Screening Group, Maastricht, 6229, GS, Netherlands. Department of Neurosciences and Behaviour, University of Sao Paulo, Ribeirao Preto, 14049-900, Brazil; National Institute of Science and Technology-Translational Medicine, Ribeirao Preto, 14049-900, Brazil. Department of Neurosciences and Behaviour, University of Sao Paulo, Ribeirao Preto, 14049-900, Brazil. Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, 05508-000, Brazil. Electronic address: yonamine@usp.br"
Journal Title:		Talanta
Year:		2021
Volume:		20201209
Issue:
Page Number:		121976 -
DOI:		10.1016/j.talanta.2020.121976
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"The present study describes the development of a novel solvent-free vortex-assisted dispersive liquid-liquid microextraction alternative based on a natural essential oil as extracting solvent (VA-EO-DLLME) for the determination of N,N-dimethyltryptamine (DMT), harmine (HRM), harmaline (HRL) and tetrahydroarmine (THH) (compounds found in the ayahuasca tea, a psychedelic plant preparation) in human plasma. After optimization through full factorial and Box-Behnken experimental designs, this VA-EO-DLLME followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was completely validated and applied to authentic plasma specimens. Sample preparation consisted in the addition of 60 mg of NaCl, 100 muL of borate buffer and 100 muL of Eucalyptus globulus essential oil to a 200 muL aliquot of human plasma. After 30 s of vortex agitation followed by 5 min of centrifugation (10,000 rpm), 80 muL of the oil supernatant was dried and resuspended in mobile phase prior to injection into the UHPLC-MS/MS system. Once optimized, the validated method yielded LoDs /= 0.9926). Intra/inter-day precision and accuracy met the acceptance criteria at three quality control (QC) levels. An additional intermediate precision study demonstrated that, except for THH and HRL at low and medium QCs, the overall method performance was similar for the three different oil sources. Matrix effect evaluation showed predominant ion suppression, ranging from 56% to 83%. Recovery varied from 33 up to 101% with an average of 50 +/- 15.8%. Selectivity studies showed no interferences. Analysis of 13 authentic samples proved method feasibility. Finally, we believe that our novel VA-EO-DLLME approach offers a very simple, fast, cost-effective and eco-friendly alternative based on the use of an easily accessible and entirely green material as an extracting solvent. This may represent an incentive for researchers to investigate novel and creative alternatives, such as essential oils, as substitutes of organic solvents for microextraction methods in forensic and clinical contexts"
Keywords:		"Carbolines Chromatography, High Pressure Liquid Humans Limit of Detection *Liquid Phase Microextraction N, N-Dimethyltryptamine *Oils, Volatile Solvents Tandem Mass Spectrometry Ayahuasca Dimethyltryptamine Dispersive liquid-liquid microextraction Essentia;neuroscience;"
Notes:		"MedlineSilveira, Gabriela de Oliveira Lourenco, Felipe Rebello Fonseca Pego, Ana Miguel Guimaraes Dos Santos, Rafael Rossi, Giordano Novak Hallak, Jaime E C Yonamine, Mauricio eng Netherlands 2021/02/18 Talanta. 2021 Apr 1; 225:121976. doi: 10.1016/j.talanta.2020.121976. Epub 2020 Dec 9"