« Previous AbstractDevelopments in the Catalytic Asymmetric Synthesis of Agrochemicals and Their Synthetic Importance    Next Abstract"Insect vectors of Leishmania: distribution, physiology and their control" »

mSphere

Title:		Fusarium graminearum Ste3 G-Protein Coupled Receptor: A Mediator of Hyphal Chemotropism and Pathogenesis
Author(s):		Sharma T; Sridhar PS; Blackman C; Foote SJ; Allingham JS; Subramaniam R; Loewen MC;
Address:		"Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada. Aquatic and Crop Resources Development Research Center, National Research Council of Canada, Ottawa, Ontario, Canada. Department of Biomedical and Molecular Science, Queen's University, Kingston, Ontario, Canada. Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada. Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada. Human Health Therapeutics Research Center, National Research Council of Canada, Ottawa, Ontario, Canada"
Journal Title:		mSphere
Year:		2022
Volume:		20221115
Issue:		6
Page Number:		e0045622 -
DOI:		10.1128/msphere.00456-22
ISSN/ISBN:		2379-5042 (Electronic) 2379-5042 (Linking)
Abstract:		"Fungal hyphal chemotropism has been shown to be a major contributor to host-pathogen interactions. Previous studies on Fusarium species have highlighted the involvement of the Ste2 G-protein-coupled receptor (GPCR) in mediating polarized hyphal growth toward host-released peroxidase. Here, the role of the opposite mating type GPCR, Ste3, is characterized with respect to Fusarium graminearum chemotropism and pathogenicity. Fgste3Delta deletion strains were found to be compromised in the chemotropic response toward peroxidase, development of lesions on germinating wheat, and infection of Arabidopsis thaliana leaves. In the absence of FgSte3 or FgSte2, F. graminearum cells exposed to peroxidase showed no phosphorylation of the cell-wall integrity, mitogen-activated protein kinase pathway component Mgv1. In addition, transcriptomic gene expression profiling yielded a list of genes involved in cellular reorganization, cell wall remodeling, and infection-mediated responses that were differentially modulated by peroxidase when FgSte3 was present. Deletion of FgSte3 yielded the downregulation of genes associated with mycotoxin biosynthesis and appressorium development, compared to the wild-type strain, both in the presence of peroxidase. Together, these findings contribute to our understanding of the mechanism underlying fungal chemotropism and pathogenesis while raising the novel hypothesis that FgSte2 and FgSte3 are interdependent on each other for the mediation of the redirection of hyphal growth in response to host-derived peroxidase. IMPORTANCE Fusarium head blight of wheat, caused by the filamentous fungus Fusarium graminearum, leads to devastating global food shortages and economic losses. Fungal hyphal chemotropism has been shown to be a major contributor to host-pathogen interactions. Here, the role of the opposite mating type GPCR, Ste3, is characterized with respect to F. graminearum chemotropism and pathogenicity. These findings contribute to our understanding of the mechanisms underlying fungal chemotropism and pathogenesis"
Keywords:		"*Fusarium/genetics Peroxidases/metabolism Mitogen-Activated Protein Kinases/genetics/metabolism Peroxidase Receptors, G-Protein-Coupled/metabolism Fusarium graminearum Fusarium head blight G-protein coupled receptors hyphal chemotropism pheromone receptor;"
Notes:		"MedlineSharma, Tanya Sridhar, Pooja S Blackman, Christopher Foote, Simon J Allingham, John S Subramaniam, Rajagopal Loewen, Michele C eng Research Support, Non-U.S. Gov't 2022/11/16 mSphere. 2022 Dec 21; 7(6):e0045622. doi: 10.1128/msphere.00456-22. Epub 2022 Nov 15"