| Title: | "Expression of MFA1 and STE6 is sufficient for mating type-independent secretion of yeast a-factor, but not mating competence" |
| Author(s): | Quinby GE; Dean JP; Deschenes RJ; |
| Address: | "Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA" |
| ISSN/ISBN: | 0172-8083 (Print) 0172-8083 (Linking) |
| Abstract: | "The yeast a-factor mating peptide and its transporter Ste6 are normally expressed only in MATa haploid cells. The a-factor is initially produced as a 36- or 38-residue peptide precursor and must undergo extensive post-translational processing to produce an active 12 amino-acid lipopeptide. To better understand the steps required for Ste6-dependent a-factor transport, we have reconstituted a-factor synthesis and transport in MATalpha haploids and MATa/alpha diploids. Ste6 and a-factor were stably expressed in MATalpha and MATa/alpha cells and the ectopically expressed a-factor was correctly processed. In addition, Ste6 was able to transport a-factor from all cell types, indicating that once expressed no other MATa-specific functions are required. However, despite significant levels of a-factor secretion, MATalpha cells are unable to support efficient mating" |
| Keywords: | "ATP-Binding Cassette Transporters/*genetics Fungal Proteins/*genetics Gene Expression Regulation, Fungal/genetics Genes, Fungal/*genetics *Genes, Mating Type, Fungal Genotype *Glycoproteins Infertility/genetics Mating Factor Peptides/*genetics Pheromones;" |
| Notes: | "MedlineQuinby, G E Dean, J P Deschenes, R J eng HL42385/HL/NHLBI NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1999/02/18 Curr Genet. 1999 Feb; 35(1):1-7. doi: 10.1007/s002940050425" |
