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Cell Calcium

Title:		"Characterization of the energy-dependent, mating factor-activated Ca2+ influx in Saccharomyces cerevisiae"
Author(s):		Prasad KR; Rosoff PM;
Address:		"Department of Pediatrics (Hematology/Oncology), Tufts University School of Medicine, Boston, Massachusetts"
Journal Title:		Cell Calcium
Year:		1992
Volume:		13
Issue:		10
Page Number:		615 - 626
DOI:		10.1016/0143-4160(92)90072-z
ISSN/ISBN:		0143-4160 (Print) 0143-4160 (Linking)
Abstract:		"The yeast mating pheromones, a and alpha factors, bind to specific G protein-coupled receptors in haploid cells and bring about both growth arrest in the early G1 phase of the cell cycle and differentiation into mating capable cells. This induces an increase in Ca2+ influx leading to elevated intracellular calcium concentrations, which has been shown to be essential for subsequent downstream events and the mating process itself [1]. We have characterized the alpha factor induced increase in cellular Ca2+ in wild type S. cerevisiae and in the temperature-sensitive cell division cycle mutants cdc7 and cdc28 which are growth-arrested at the G0-G1 border at the nonpermissive temperature. We observed a 2-4 fold increase in the initial velocity of Ca2+ influx in alpha factor-treated wild-type cells and in cdc7 and cdc28 cells grown at the nonpermissive temperature. Calcium influx was energy dependent, inhibited by membrane depolarization and slightly increased by hyperpolarization. Furthermore, Ca2+ influx was sensitive to both divalent and trivalent cations, but was unaffected by nifedipine and verapamil. These data demonstrate that budding yeast possesses a regulated Ca2+ transport mechanism, the activation of which is dependent upon exit out of the cell cycle and growth cessation. This transport mechanism has many similarities to that observed in mitogen-stimulated mammalian cells"
Keywords:		"2, 4-Dinitrophenol Biological Transport, Active/drug effects CDC28 Protein Kinase, S cerevisiae/metabolism Calcium/*metabolism Calcium Channels/drug effects Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Cations/pharmacology *Cell Cycle Proteins Ce;"
Notes:		"MedlinePrasad, K R Rosoff, P M eng Research Support, Non-U.S. Gov't Netherlands 1992/11/01 Cell Calcium. 1992 Nov; 13(10):615-26. doi: 10.1016/0143-4160(92)90072-z"