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Comp Biochem Physiol B Biochem Mol Biol

Title:		Identification and ligand binding of a chemosensory protein from sea louse Caligus rogercresseyi (Crustacea: Copepoda)
Author(s):		Pino J; Godoy R; Venthur H; Larama G; Quiroz A; Mutis A;
Address:		"Cargill Innovation Center Colaco, Chile. Electronic address: jorge_pino@cargill.com. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Temuco, Chile; Departamento de Ciencias Quimicas y Recursos Naturales, Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Chile. Electronic address: r.godoy04@ufromail.cl. Departamento de Ciencias Quimicas y Recursos Naturales, Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Chile; Centro de Investigacion Biotecnologica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, Chile. Electronic address: herbert.venthur@ufrontera.cl. Centro de Genomica Nutricional Agroacuicola, CGNA, Temuco, Chile. Electronic address: giovanni.larama@cgna.cl. Departamento de Ciencias Quimicas y Recursos Naturales, Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Chile; Centro de Investigacion Biotecnologica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, Chile. Electronic address: andres.quiroz@ufrontera.cl. Departamento de Ciencias Quimicas y Recursos Naturales, Facultad de Ingenieria y Ciencias, Universidad de La Frontera, Chile; Centro de Investigacion Biotecnologica Aplicada al Medio Ambiente, CIBAMA, Universidad de La Frontera, Temuco, Chile. Electronic address: ana.mutis@ufrontera.cl"
Journal Title:		Comp Biochem Physiol B Biochem Mol Biol
Year:		2023
Volume:		20230114
Issue:
Page Number:		110830 -
DOI:		10.1016/j.cbpb.2023.110830
ISSN/ISBN:		1879-1107 (Electronic) 1096-4959 (Linking)
Abstract:		"Caligus rogercresseyi is an ectoparasitic copepod that negatively affects the salmon farming industry, causing economic losses. To use phytochemicals as feed additives, or other chemicals that could elicit behavioral responses in C. rogercresseyi, the chemosensory recognition process is crucial. Therefore, to establish how C. rogercresseyi recognizes glucosinolates and their derivates isothiocyanates, a chemosensory protein (CSP) described as specific carrier of these chemicals in sea louse (CrogCSP) was identified in this study. The recombinant CSP and its selectivity against different chemical compounds was tested by fluorescence binding assays. Phylogenetic analysis revealed a close relationship among CrogCSP and other reported CSPs. Our results indicate that phenyl isothiocyanate and isophorone exhibited dissociation constants of 4.17 and 4.28 muM of K(i), respectively, indicating affinity over other chemicals, such as fatty acids and sinigrin. Structural findings suggest a unique binding site capable of accept several types of chemicals, similar to what has been reported for crystallized insect CSPs. Finally, this study lays the foundation for a deeper understanding of CSPs in crustaceans and especially in C. rogercresseyi. Likewise, the identification of chemosensory proteins could serve as the first step towards novel semiochemicals discovery to being applied in the sea louse controlling"
Keywords:		Animals *Copepoda *Phthiraptera Phylogeny Ligands *Fish Diseases/parasitology Chemosensory proteins (CSPs) Glucosinolates Olfaction Reverse chemical ecology Sea louse;
Notes:		"MedlinePino, Jorge Godoy, Ricardo Venthur, Herbert Larama, Giovanni Quiroz, Andres Mutis, Ana eng England 2023/01/18 Comp Biochem Physiol B Biochem Mol Biol. 2023 Apr-May; 265:110830. doi: 10.1016/j.cbpb.2023.110830. Epub 2023 Jan 14"