| Title: | Comparing the effects of unconventional and conventional crude oil exposures on zebrafish and their progeny using behavioral and genetic markers |
| Author(s): | Philibert DA; Lyons DD; Tierney KB; |
| Address: | "Huntsman Marine Science Centre, St. Andrews, New Brunswick E5B 2L7, Canada; Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada. Electronic address: philiber@ualberta.ca. Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada. Huntsman Marine Science Centre, St. Andrews, New Brunswick E5B 2L7, Canada; School of Public Health, University of Alberta, Edmonton, Alberta T6G 2E9, Canada" |
| DOI: | 10.1016/j.scitotenv.2020.144745 |
| ISSN/ISBN: | 1879-1026 (Electronic) 0048-9697 (Linking) |
| Abstract: | "Diluted bitumen, also known as dilbit, is transported by rail and pipeline across Canada and the United States. Due to the fewer number of studies characterizing the toxicity of dilbit, a dilbit spill poses an unknown risk to freshwater aquatic ecosystems. In the following study, we compared the impact of early-life exposure to conventional and unconventional crude oils on the optomotor behavior, reproductive success, and transgenerational differences in gene expression in zebrafish and their progeny. For exposures, water accommodated fractions (WAFs) of crude oil were generated using a 1:1000 oil to water ratio for 3 different crudes; mixed sweet blend (MSB), medium sour composite (MSC) and dilbit. All three oils generated unique volatile organic compound (VOC) and polycyclic aromatic compound (PAC) profiles. Of the WAFs tested, only dilbit decreased the eye size of 2 dpf larvae, and only MSB exposed larvae had an altered behavioral response to a visual simulation of a predator. Early-life exposure to crude oil had no lasting impact on reproductive success of adult fish; however, each oil had unique impacts on the basal gene expression of the somatically exposed offspring. In this study, the biological effects differed between each of the oils tested, which implied chemical composition plays a critical role in determining the sublethal toxicity of conventional and unconventional crude oils in freshwater ecosystems" |
| Keywords: | "Animals Canada Ecosystem Genetic Markers *Petroleum/analysis/toxicity *Petroleum Pollution/adverse effects *Polycyclic Aromatic Hydrocarbons *Water Pollutants, Chemical/toxicity Zebrafish Aquatic toxicology Crude oil Dilbit Fish behavior Transgenic effect;" |
| Notes: | "MedlinePhilibert, Danielle A Lyons, Danielle D Tierney, Ketih B eng Netherlands 2021/03/20 Sci Total Environ. 2021 May 20; 770:144745. doi: 10.1016/j.scitotenv.2020.144745. Epub 2021 Jan 23" |
